Abnormality of CD4+CD25+ regulatory T cells in idiopathic thrombocytopenic purpura

被引：201
作者：
Liu, Bin
Zhao, Hui
Poon, Man-Chiu
Han, Zhibo
Gu, Dongsheng
Xu, Maoqiang
Jia, Hairong
Yang, Renchi
Han, Zhong Chao
机构：
[1] Chinese Acad Med Sci, Inst Hematol, State Key Lab Expt Hematol, Tianjin 300020, Peoples R China
[2] Peking Union Med Coll, Tianjin 300020, Peoples R China
[3] Chinese Acad Med Sci, Blood Dis Hosp, Tianjin 300020, Peoples R China
[4] Natl Res Ctr Stem Cell Engn & Technol, Tianjin, Peoples R China
[5] Univ Calgary, Dept Med, Div Hematol & Hematol Malignancies, Calgary, AB, Canada
来源：
EUROPEAN JOURNAL OF HAEMATOLOGY | 2007年 / 78卷 / 02期
关键词：
idiopathic thrombocytopenic purpura; regulatory T cells; immunosuppression;
D O I：
10.1111/j.1600-0609.2006.00780.x
中图分类号：
R5 [内科学];
学科分类号：
1002 ; 100201 ;
摘要：
Objectives: The aim of this study was to explore the profile and function of CD4(+)CD25(+) regulatory T cells (Treg cells) in idiopathic thrombocytopenic purpura (ITP) patients. Methods: Treg cell numbers were analyzed by flow cytometric analysis in peripheral blood mononuclear cells collected from healthy donors or patients with ITP. Quantification of cell proliferation was assayed by an enzyme-linked immunosorbent assay kit, based on the measurement of BrdU incorporation during DNA synthesis. Results: The percentage of Treg cells was significantly decreased in ITP patients in active and non-remission state(5.79 +/- 1.22%) when compared with the patients in remission(11.63 +/- 4.56%) and to healthy subjects(12.68 +/- 3.59%). The suppressive activity of Treg cells in ITP patients was also found to be impaired. Conclusion: These results suggest that decreased number and function of Treg cells might be one of mechanisms that cause immune regulation dysfunction in ITP.
引用
收藏
页码：139 / 143
页数：5
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