Microchip-Based Capillary Electrophoresis for DNA Analysis in Modern Biotechnology: A Review

被引：20
作者：
Kailasa, Suresh Kumar
Kang, Seong Ho [1 ]
机构：
[1] Chonbuk Natl Univ, Dept Chem, Jeonju 561756, South Korea
来源：
SEPARATION AND PURIFICATION REVIEWS | 2009年 / 38卷 / 03期
关键词：
Microchip electrophoresis; DNA; programmed field strength gradients; genetically modified organisms; POLYMERASE-CHAIN-REACTION; FLIGHT-MASS-SPECTROMETRY; LASER DESORPTION/IONIZATION-TIME; DENSITY NANOVIAL ARRAYS; TOTAL ANALYSIS SYSTEM; SINGLE-CELL ANALYSIS; DOUBLE-STRANDED DNA; ELECTROCHEMICAL DETECTION; FLUORESCENCE DETECTION; SAMPLE PREPARATION;
D O I：
10.1080/15422110903095136
中图分类号：
O69 [应用化学];
学科分类号：
081704 ;
摘要：
Microchip-based capillary electrophoresis (ME) raises fascinating possibilities for new analytical technologies particularly in the detection of DNA fragments in modern biology. The ME technique has gained considerable popularity with significant progress being made in the development of miniaturized microfluidic devices in micro-volume analysis methods. The ME offers the potential for highly efficient, simultaneous analysis of a large number of biologically important molecules in genomic, proteomic and genetically modified organisms (GMOs) in food stuffs. Recently, the ME technique has greatly impacted biotechnological research with potential applications in smart devices that can operate at the level of molecular manipulation. This review is focused on recent developments and applications of ME to the detection and separation of DNA fragments in various molecules i.e., biotechnological and clinical research.
引用
收藏
页码：242 / 288
页数：47
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