Genetic analysis of enterovirus 71 isolated from fatal and non-fatal cases of hand, foot and mouth disease during an epidemic in Taiwan, 1998

被引:137
作者
Shih, SR
Ho, MS
Lin, KH
Wu, SL
Chen, YT
Wu, CN
Lin, TY
Chang, LY
Tsao, KC
Ning, HC
Chang, PY
Jung, SM
Hsueh, C
Chang, KS
机构
[1] Chang Gung Univ, Sch Med Technol, Tao Yuan 333, Taiwan
[2] Chang Gung Mem Hosp, Dept Clin Pathol, Tao Yuan, Taiwan
[3] Acad Sinica, Inst Biomed Sci, Taipei 115, Taiwan
[4] Kaohsiung Med Coll, Virol Lab, Kaohsiung, Taiwan
[5] Chang Gung Childrens Hosp, Dept Infect Dis, Tao Yuan, Taiwan
[6] Chang Gung Mem Hosp, Dept Pathol, Tao Yuan, Taiwan
[7] Chang Gung Univ, Grad Inst Clin Med, Tao Yuan 333, Taiwan
关键词
enterovirus; 71; hand; foot and mouth disease; Taiwan;
D O I
10.1016/S0168-1702(00)00162-3
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
A Large scale outbreak of hand-foot-and-month disease (HFMD) occurred in Taiwan in 1998, in which more than 80 children died of shock syndrome with pulmonary edema/hernorrhage. Enterovirus 71 was implicated as the cause of this outbreak. In order to understand the virological basis responsible for mortality on this scale, nucleotide sequences of VP1 that is important for serotypic specificity, and the S'-non-coding region (5'-NCR) that is important for replication efficiency, were analyzed comparatively. Phylogenetic analysis of both VPI and 5'-NCR of nine EV71 isolates derived from specimens of fatal patients and seven isolates derived from uncomplicated HFMD patients showed that all but one isolate fell into genotype B. The one distinct isolate From a case of uncomplicated HFMD belonged to genotype C that was clustered along with one isolate from Taiwan in 1986. Complete sequence analysis of two selected isolates, one from the spinal cord of a fatal case and one from the vesicle fluid of a patient with mild HFMD, confirmed a high degree (97-100%) of identity in nucleotide sequence throughout the entire genome, except focal regions of 3C and 3'-NCR where the nucleotide homology was 90-91%. The identity of the deduced amino acid sequence in the 3C region that encodes viral proteinase dropped further to 86%. a result of missense mutations at the first nucleotide position of many codons. (C) 2000 Elsevier Science B.V. All rights reserved.
引用
收藏
页码:127 / 136
页数:10
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