Dual channel mitochondria-targeted fluorescent probe for detection of nitric oxide in living cells and zebrafish

被引：10

作者：

Su, Wanting ^{[1
]}

Huang, Ling ^{[1
]}

Liang, Xing ^{[1
]}

Zhu, Lin ^{[1
]}

Lin, Weiying ^{[1
]}

机构：

[1] Guangxi Univ, Sch Chem & Chem Engn, Inst Opt Mat & Chem Biol, Guangxi Key Lab Electrochem Energy Mat, Nanning 530004, Guangxi, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2021年 / 412卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Fluorescence sensing; Fluorescence imaging; ADENINE-NUCLEOTIDE TRANSLOCASE; SODIUM-NITROPRUSSIDE; REACTIVE OXYGEN; PEROXYNITRITE; SYSTEM;

D O I：

10.1016/j.jphotochem.2021.113256

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel dual channel mitochondria-targeted fluorescent probe has been developed to detect nitric oxide (NO), an important and short-life reactive nitrogen species (RNS) which mainly generates in the mitochondria. The new probe Mi-NO has exhibited dual emission property as well as unique spectrum responses towards NO. Upon the addition of NO, the fluorescence of Mi-NO quenched significantly. Molecular docking calculations suggest a potential molecular target for Mi-NO in the mitochondria protein adenine nucleotide translocator (ANT). High colocalization coefficient also suggested that Mi-NO can effectively target mitochondria. Moreover, Mi-NO has been successfully employed in imaging NO in living cells and zebrafish, affording a potential for studying the biological functions of NO in living systems.

引用

页数：8

共 39 条

[31] A fluorescent and colorimetric chemosensor for nitric oxide based on 1,8-naphthalimide [J].

Wang, Min ;

Xu, Zhaochao ;

Wang, Xu ;

Cui, Jingnan .

DYES AND PIGMENTS, 2013, 96 (02) :333-337

[32] A naked-eye and ratiometric near-infrared probe for palladium via modulation of a π-conjugated system of cyanines [J].

Wang, Xiaohang ;

Guo, Zhiqian ;

Zhu, Shiqin ;

Tian, He ;

Zhu, Weihong .

CHEMICAL COMMUNICATIONS, 2014, 50 (88) :13525-13528

[33] Mitochondrial fusion and fission in cell life and death [J].

Westermann, Benedikt .

NATURE REVIEWS MOLECULAR CELL BIOLOGY, 2010, 11 (12) :872-884

[34] Sensitivity evaluation of rhodamine B hydrazide towards nitric oxide and its application for macrophage cells imaging [J].

Wu, Chi-Ming ;

Chen, Yen-Hao ;

Dayananda, Kasala ;

Shiue, Tsun-Wei ;

Hung, Chen-Hsiung ;

Liaw, Wen-Feng ;

Chen, Po-Yu ;

Wang, Yun-Ming .

ANALYTICA CHIMICA ACTA, 2011, 708 (1-2) :141-148

[35] A Highly Selective Low-Background Fluorescent Imaging Agent for Nitric Oxide [J].

Yang, Youjun ;

Seidlits, Stephanie K. ;

Adams, Michelle M. ;

Lynch, Vincent M. ;

Schmidt, Christine E. ;

Anslyn, Eric V. ;

Shear, Jason B. .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 2010, 132 (38) :13114-13116

[36] Far-red to near infrared analyte-responsive fluorescent probes based on organic fluorophore platforms for fluorescence imaging [J].

Yuan, Lin ;

Lin, Weiying ;

Zheng, Kaibo ;

He, Longwei ;

Huang, Weimin .

CHEMICAL SOCIETY REVIEWS, 2013, 42 (02) :622-661

[37] A fast-responsive fluorescent probe for detection of gold ions in water and synthetic products [J].

Yuan, Lin ;

Lin, Weiying ;

Yang, Yueting ;

Song, Jizeng .

CHEMICAL COMMUNICATIONS, 2011, 47 (16) :4703-4705

[38] Adenine nucleotide translocase: a component of the phylogenetically conserved cell death machinery [J].

Zhivotovsky, B. ;

Galluzzi, L. ;

Kepp, O. ;

Kroemer, G. .

CELL DEATH AND DIFFERENTIATION, 2009, 16 (11) :1419-1425

[39] A ratiometric mitochondria-targeting two-photon fluorescent probe for imaging of nitric oxide in vivo [J].

Zhu, Xinyue ;

Chen, Jin-Quan ;

Ma, Chen ;

Liu, Xiaoyan ;

Cao, Xiao-Ping ;

Zhang, Haixia .

ANALYST, 2017, 142 (24) :4623-4628

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