Dual channel mitochondria-targeted fluorescent probe for detection of nitric oxide in living cells and zebrafish

被引：10

作者：

Su, Wanting ^{[1
]}

Huang, Ling ^{[1
]}

Liang, Xing ^{[1
]}

Zhu, Lin ^{[1
]}

Lin, Weiying ^{[1
]}

机构：

[1] Guangxi Univ, Sch Chem & Chem Engn, Inst Opt Mat & Chem Biol, Guangxi Key Lab Electrochem Energy Mat, Nanning 530004, Guangxi, Peoples R China

来源：

JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY | 2021年 / 412卷

基金：

中国国家自然科学基金;

关键词：

Fluorescent probe; Fluorescence sensing; Fluorescence imaging; ADENINE-NUCLEOTIDE TRANSLOCASE; SODIUM-NITROPRUSSIDE; REACTIVE OXYGEN; PEROXYNITRITE; SYSTEM;

D O I：

10.1016/j.jphotochem.2021.113256

中图分类号：

O64 [物理化学（理论化学）、化学物理学];

学科分类号：

070304 ; 081704 ;

摘要：

A novel dual channel mitochondria-targeted fluorescent probe has been developed to detect nitric oxide (NO), an important and short-life reactive nitrogen species (RNS) which mainly generates in the mitochondria. The new probe Mi-NO has exhibited dual emission property as well as unique spectrum responses towards NO. Upon the addition of NO, the fluorescence of Mi-NO quenched significantly. Molecular docking calculations suggest a potential molecular target for Mi-NO in the mitochondria protein adenine nucleotide translocator (ANT). High colocalization coefficient also suggested that Mi-NO can effectively target mitochondria. Moreover, Mi-NO has been successfully employed in imaging NO in living cells and zebrafish, affording a potential for studying the biological functions of NO in living systems.

引用

页数：8

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