Arachidonic acid stimulates internalisation of leptin by human placental choriocarcinoma (BeWo) cells

被引：3
作者：
Duttaroy, AK
Taylor, J
Gordon, MJ
Hoggard, N
Campbell, FM
机构：
[1] Univ Oslo, Inst Nutr Res, N-0316 Oslo, Norway
[2] Rowett Res Inst, Aberdeen AB21 9SB, Scotland
[3] Univ Alberta, Cardiovasc Res Grp, Edmonton, AB T6G 2M7, Canada
来源：
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2002年 / 299卷 / 03期
关键词：
D O I：
10.1016/S0006-291X(02)02647-5
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Arachidonic acid at 100 nM stimulated internalisation of I-125-leptin in human placental choriocarcinoma (BeWo) cells by Mold compared with controls. In contrast, eicosapentaenoic acid at similar concentration decreased internalisation of leptin by 2-fold. Use of ibuprofen and indomethacin (inhibitors of prostaglandin synthesis) inhibited the stimulatory effect of arachidonic acid. Prostaglandin E-2, a cyclooxygenase metabolite of arachidonic acid, stimulated internalisation of leptin by these cells. All these data demonstrate that stimulation of leptin internalisation by arachidonic acid in placental trophoblasts may be mediated via prostaglandin E2. (C) 2002 Elsevier Science (USA). All rights reserved.
引用
收藏
页码：432 / 437
页数：6
相关论文
共 39 条
  • [1] Placental leptin
    Ashworth, CJ
    Hoggard, N
    Thomas, L
    Mercer, JG
    Wallace, JM
    Lea, RG
    [J]. REVIEWS OF REPRODUCTION, 2000, 5 (01): : 18 - 24
  • [2] Subcellular localization and internalization of the four human leptin receptor isoforms
    Barr, VA
    Lane, K
    Taylor, SI
    [J]. JOURNAL OF BIOLOGICAL CHEMISTRY, 1999, 274 (30) : 21416 - 21424
  • [3] Interaction of free fatty acids with human leptin
    Campbell, FM
    Gordon, MJ
    Hoggard, N
    Dutta-Roy, AK
    [J]. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 1998, 247 (03) : 654 - 658
  • [4] PLASMA-MEMBRANE FATTY-ACID-BINDING PROTEIN (FABP(PM)) IS EXCLUSIVELY LOCATED IN THE MATERNAL FACING MEMBRANES OF THE HUMAN PLACENTA
    CAMPBELL, FM
    DUTTAROY, AK
    [J]. FEBS LETTERS, 1995, 375 (03) : 227 - 230
  • [5] Campbell FM, 1997, J LIPID RES, V38, P2558
  • [6] INHIBITORS OF CYTOCHROME P-450-DEPENDENT ARACHIDONIC-ACID METABOLISM
    CAPDEVILA, J
    GIL, L
    ORELLANA, M
    MARNETT, LJ
    MASON, JI
    YADAGIRI, P
    FALCK, JR
    [J]. ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 1988, 261 (02) : 257 - 263
  • [7] Evidence that the diabetes gene encodes the leptin receptor: Identification of a mutation in the leptin receptor gene in db/db mice
    Chen, H
    Charlat, O
    Tartaglia, LA
    Woolf, EA
    Weng, X
    Ellis, SJ
    Lakey, ND
    Culpepper, J
    Moore, KJ
    Breitbart, RE
    Duyk, GM
    Tepper, RI
    Morgenstern, JP
    [J]. CELL, 1996, 84 (03) : 491 - 495
  • [8] Phenotypes of mouse diabetes and rat fatty due to mutations in the OB (leptin) receptor
    Chua, SC
    Chung, WK
    WuPeng, XS
    Zhang, YY
    Liu, SM
    Tartaglia, L
    Leibel, RL
    [J]. SCIENCE, 1996, 271 (5251) : 994 - 996
  • [9] COLEMAN RA, 1994, PHARMACOL REV, V46, P205
  • [10] Pharmacological characterization of [3H]-prostaglandin E2 binding to the cloned human EP4 prostanoid receptor
    Davis, TL
    Sharif, NA
    [J]. BRITISH JOURNAL OF PHARMACOLOGY, 2000, 130 (08) : 1919 - 1926
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