Enzyme-free amplified detection of microRNA using target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme

被引：13
作者：
Chen, Jinfeng [1 ,2 ,3 ,5 ]
Liu, Bo [1 ,2 ,3 ]
Song, Xiaorong [1 ,2 ,3 ]
Tong, Ping [1 ,2 ,3 ,4 ]
Yang, Huanghao [1 ,2 ,3 ,6 ]
Zhang, Lan [1 ,2 ,3 ,4 ]
机构：
[1] Fuzhou Univ, Key Lab Anal & Detect Food Safety, Minist Educ, Fuzhou 350108, Peoples R China
[2] Fuzhou Univ, Key Lab Anal & Detect Food Safety Fujian Prov, Fuzhou 350108, Peoples R China
[3] Fuzhou Univ, Coll Chem, Fuzhou 350108, Peoples R China
[4] Fuzhou Univ, Testing Ctr, Sport Sci Res Ctr, Fuzhou 350002, Peoples R China
[5] Longyan Entry Exit Inspect & Quarantine Bur PRC, Longyan 364000, Peoples R China
[6] Yanbian Univ, Minist Educ, Key Lab Nat Resources Changbai Mt & Funct Mol, Yanji 133002, Peoples R China
来源：
SCIENCE CHINA-CHEMISTRY | 2015年 / 58卷 / 12期
基金：
中国国家自然科学基金;
关键词：
microRNA; enzyme-free amplified detection; target-catalyzed hairpin assembly; magnesium ion-dependent deoxyribozyme; SENSITIVE DETECTION; FREE AMPLIFICATION; DNA; CIRCUITS; SENSOR;
D O I：
10.1007/s11426-015-5391-0
中图分类号：
O6 [化学];
学科分类号：
0703 ;
摘要：
As microRNAs (miRNAs) are aberrantly expressed in a variety of cancers, detecting them precisely is of great importance. Here we constructed a sensitive and selective enzyme-free sensing platform for miRNA detection based on target-catalyzed hairpin assembly and magnesium ion-dependent deoxyribozyme (Mg2+-dependent DNAzyme). This sensing method introduces two amplification circuits simultaneously and shows a low detection limit of 1 pmol/L. This enzyme-free method is especially preferred because of its facility and economy. Furthermore, this amplified sensor shows high selectivity for discriminating perfectly complementary target and other mismatched RNAs. Therefore, the established strategy could be used as a simple, sensitive and selective method for target miRNA detection.
引用
收藏
页码：1906 / 1911
页数：6
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