Flavonoids of Herba Epimedii regulate osteogenesis of human mesenchymal stem cells through BMP and Wnt/β-catenin signaling pathway

被引:118
作者
Zhang, Jin-fang [1 ,2 ,4 ]
Li, Guo [4 ]
Chan, Chu-yan [4 ]
Meng, Chun-ling [4 ]
Lin, Marie Chia-mi [3 ]
Chen, Yang-chao [4 ]
He, Ming-liang [4 ]
Leung, Ping-chung [5 ]
Kung, Hsiang-fu [2 ,4 ]
机构
[1] Tsinghua Univ, Dept Biol Sci & Biotechnol, Beijing 100084, Peoples R China
[2] Tsinghua Univ, Grad Sch Shenzhen, Div Life Sci, Shenzhen 518055, Peoples R China
[3] Univ Hong Kong, Dept Chem, Integrat Chem Biol Lab, Inst Mol Technol, Pokfulam, Hong Kong, Peoples R China
[4] Chinese Univ Hong Kong, Stanley Ho Ctr Emerging Infect Dis, Hong Kong, Hong Kong, Peoples R China
[5] Chinese Univ Hong Kong, Dept Orthopaed & Traumatol, Hong Kong, Hong Kong, Peoples R China
关键词
BMP signaling pathway; Wnt/beta-catenin signaling pathway; Flavonoids of Herb Epidemium; Mesenchymal stem cells; Osteogenic differentiation; OSTEOBLAST DIFFERENTIATION; KOREANUM NAKAI; PROLIFERATION; SUPPRESSION; EXPRESSION; THERAPY; OSTERIX; GENE;
D O I
10.1016/j.mce.2009.08.012
中图分类号
Q2 [细胞生物学];
学科分类号
071009 ; 090102 ;
摘要
Herba Epimedii is one of the most commonly used Chinese herbs for treating osteoporosis. In the present study, the flavonoids of Herba Epimedii (HEF) have shown to promote the osteogenic differentiation of human bone marrow-derived mesenchymal stem cells. They were noted to enhance the mRNA expression of BMP-2, BMP-4, Runx2, beta-catenin and cyclinD1, all of which are BMP or Wnt-signaling pathway related regulators. The osteogenic effect was inhibited by the introduction of noggin and DKK-1, which is classical inhibitor of BMP and Wnt/beta-catenin signaling, respectively. These results suggest that HEF exerts promoting effect on osteogenic differentiation, which plausibly functions via the BMP and Wnt/beta-catenin signaling pathways. Considering the therapeutic efficiency and economical issues, HEF may be a potential candidate for promoting bone regeneration. On the other hand, osteogenic differentiation of MSCs may also be a promising and attractive tool to apply in bone repair. (C) 2009 Published by Elsevier Ireland Ltd.
引用
收藏
页码:70 / 74
页数:5
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