Therapeutic glycoprotein production in mammalian cells

被引：210

作者：

Lalonde, Marie-Eve ^{[1
]}

Durocher, Yves ^{[1
,2
]}

机构：

[1] Univ Montreal, Fac Med, Dept Biochim & Med Mol, Montreal, PQ H3C 3J7, Canada

[2] Natl Res Council Canada, Life Sci, Human Hlth Therapeut Portfolio, Montreal, PQ H4P 2R2, Canada

来源：

JOURNAL OF BIOTECHNOLOGY | 2017年 / 251卷

基金：

加拿大健康研究院;

关键词：

Glycoprotein; Cell engineering; Glycoengineering; CHO cells; Biotherapeutics; CHINESE-HAMSTER OVARY; RECOMBINANT FACTOR-VIII; N-GLYCOLYLNEURAMINIC ACID; HUMAN MONOCLONAL-ANTIBODY; DEPENDENT CELLULAR CYTOTOXICITY; MATRIX ATTACHMENT REGIONS; LARGE-SCALE TRANSFECTION; LOW CULTURE TEMPERATURE; COAGULATION-FACTOR VIII; HUMAN ANTITHROMBIN-III;

D O I：

10.1016/j.jbiotec.2017.04.028

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Over the last years, the biopharmaceutical industry has significantly turned its biologics production towards mammalian cell expression systems. The presence of glycosylation machineries within these systems, and the fact that monoclonal antibodies represent today the vast majority of new therapeutic candidates, has largely influenced this new direction. Recombinant glycoproteins, including monoclonal antibodies, have shown different biological properties based on their glycan profiles. Thus, the industry has developed cell engineering strategies not only to improve cell's specific productivity, but also to adapt their glycosylation profiles for increased therapeutic activity. Additionally, the advance of "omics" technologies has recently given rise to new possibilities in improving these expression platforms and will significantly help developing new strategies, in particular for CHO (Chinese Hamster Ovary) cells.

引用

页码：128 / 140

页数：13

共 259 条

[1] Effect of Culture Temperature on Erythropoietin Production and Glycosylation in a Perfusion Culture of Recombinant CHO Cells [J].

Ahn, Woo Suk ;

Jeon, Jae-Jin ;

Jeong, Yeong-Ran ;

Lee, Seung Joo ;

Yoon, Sung Kwan .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 101 (06) :1234-1244

[2] Correlation of DNA methylation with histone modifications across the HNRPA2B1-CBX3 ubiquitously-acting chromatin open element (UCOE) [J].

Allen, Marianne Lindahl ;

Antoniou, Michael .

EPIGENETICS, 2007, 2 (04) :227-236

[3] Identification of novel small molecule enhancers of protein production by cultured mammalian cells [J].

Allen, Martin J. ;

Boyce, James P. ;

Trentalange, Michael T. ;

Treiber, David L. ;

Rasmussen, Brian ;

Tillotson, Benjamin ;

Davis, Raymond ;

Reddy, Pranhitha .

BIOTECHNOLOGY AND BIOENGINEERING, 2008, 100 (06) :1193-1204

[4]

Andersen MR, 2009, ENCY IND BIOTECHNOLO

[5] Molecular Characterization of a Human Matrix Attachment Region Epigenetic Regulator [J].

Arope, Salina ;

Harraghy, Niamh ;

Pjanic, Milos ;

Mermod, Nicolas .

PLOS ONE, 2013, 8 (11)

[6] CARBOHYDRATE-SPECIFIC RECEPTORS OF THE LIVER [J].

ASHWELL, G ;

HARFORD, J .

ANNUAL REVIEW OF BIOCHEMISTRY, 1982, 51 :531-554

[7] Metabolic control of recombinant protein N-glycan processing in NS0 and CHO cells [J].

Baker, KN ;

Rendall, MH ;

Hills, AE ;

Hoare, M ;

Freedman, RB ;

James, DC .

BIOTECHNOLOGY AND BIOENGINEERING, 2001, 73 (03) :188-202

[8] First administration to humans of a monoclonal antibody cocktail against rabies virus: Safety, tolerability, and neutralizing activity [J].

Bakker, A. B. H. ;

Python, C. ;

Kissling, C. J. ;

Pandya, P. ;

Marissen, W. E. ;

Brink, M. F. ;

Lagerwerf, F. ;

Worst, S. ;

van Corven, E. ;

Kostense, S. ;

Hartmann, K. ;

Weverling, G. J. ;

Uytdehaag, F. ;

Herzog, C. ;

Briggs, D. J. ;

Rupprecht, C. E. ;

Grimaldi, R. ;

Goudsmit, J. .

VACCINE, 2008, 26 (47) :5922-5927

[9]

Baldi L, 2012, METHODS MOL BIOL, V801, P13, DOI 10.1007/978-1-61779-352-3_2

[10] Recent advances in mammalian protein production [J].

Bandaranayake, Ashok D. ;

Almo, Steven C. .

FEBS LETTERS, 2014, 588 (02) :253-260

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