Immunomodulation by duck defensin, Apl_AvBD2: In vitro dendritic cell immunoreceptor (DCIR) mRNA suppression, and B- and T-lymphocyte chemotaxis

被引：14

作者：

Soman, Soja Saghar ^{[1
]}

Nair, Sajith ^{[1
]}

Issac, Aneesh ^{[1
]}

Arathy, D. S. ^{[1
]}

Niyas, K. P. ^{[1
]}

Anoop, M. ^{[1
]}

Sreekumar, E. ^{[1
]}

机构：

[1] RGCB, Mol Virol Lab, Dept Mol Microbiol, Thiruvananthapuram 695014, Kerala, India

来源：

MOLECULAR IMMUNOLOGY | 2009年 / 46卷 / 15期

关键词：

Avian; Defensin; Chemotaxis; DCIR; Immunomodulation; ANTIMICROBIAL PEPTIDES; GENOMIC ORGANIZATION; VACCINE DEVELOPMENT; ANAS-PLATYRHYNCHOS; INFLUENZA; EXPRESSION; BETA-DEFENSIN-3; CHEMOKINES; ADJUVANTS; IMMUNITY;

D O I：

10.1016/j.molimm.2009.06.003

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The present study analyzed the immunomodulatory potential of a newly identified duck P-defensin, Apl_AvBD2. Recombinant Apl_AvBD2 expressed in HEK293T cells induced a concentration dependent in vitro migration of duck splenocytes, and spleen B- and T-lymphocytes, which was specifically inhibited by anti-Apl-AvBD2 polyclonal antibodies. Among the transcripts of 13 immunologically important genes analyzed in cultured splenocytes for the early immunomodulatory effect of Apl-AvBD2, dendritic cell immunoreceptor (DCIR) mRNA was found to be significantly down-regulated. However, there were no major changes in the expression levels of transcripts for cell surface proteins (MHC I, MHC II 2 beta-chain, TCR-beta, TLR-7, DCAR, CD44, and CD58) and cytokines (IL-2, IFN-gamma, RANTES, MIP-1 beta-like and MCP-1 like chemokines). Our results reveal chemotactic and immunomodulatory properties of Apl_AvBD2, two important functions that would help in employing this protein as a molecular adjuvant with avian vaccines. (C) 2009 Elsevier Ltd. All rights reserved.

引用

页码：3070 / 3075

页数：6

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