Nickel-inducible lysis system in Synechocystis sp PCC 6803

被引：73

作者：

Liu, Xinyao

Curtiss, Roy, III ^{[1
]}

机构：

[1] Arizona State Univ, Ctr Infect Dis & Vaccinol, Biodesign Inst, Tempe, AZ 85287 USA

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 2009年 / 106卷 / 51期

关键词：

biofuel; phage lysis genes; SP PCC-6803; STRAIN; MICROORGANISMS; EXPRESSION; ENDOLYSIN; PROTEINS; DYNAMICS; HOLINS; PHASE;

D O I：

10.1073/pnas.0911953106

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

We designed and constructed a controllable inducing lysis system in Synechocystis sp. PCC 6803 to facilitate extracting lipids for biofuel production. Several bacteriophage-derived lysis genes were integrated into the genome and placed downstream of a nickel-inducible signal transduction system. We applied 3 strategies: (i) directly using the phage lysis cassette, (ii) constitutively expressing endolysin genes while restricting holin genes, and (iii) combining lysis genes from different phages. Significant autolysis was induced in the Synechocystis sp. PCC 6803 cells with this system by the addition of NiSO4. Our inducible cyanobacterial lysing system eliminates the need for mechanical or chemical cell breakage and could facilitate recovery of biofuel from cyanobacteria.

引用

收藏

页码：21550 / 21554

页数：5

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