Development of Ganoderma lucidum spore powder based proteoglycan and its application in hyperglycemic, antitumor and antioxidant function

被引：22
作者：
Zhu, Li-Fang [1 ,2 ]
Yao, Yuanfa [1 ,2 ]
Ahmad, Zeeshan [3 ]
Chang, Ming-Wei [1 ,2 ,4 ]
机构：
[1] Zhejiang Univ, Key Lab Biomed Engn, Educ Minist China, Hangzhou 310027, Zhejiang, Peoples R China
[2] Zhejiang Univ, Zhejiang Prov Key Lab Cardiocerebral Vasc Detect, Hangzhou 310027, Zhejiang, Peoples R China
[3] De Montfort Univ, Leicester Sch Pharm, Gateway, Leicester LE1 9BH, Leics, England
[4] Univ Ulster, Nanotechnol & Integrated Bioengn Ctr, Jordanstown Campus, Newtownabbey BT37 0QB, North Ireland
来源：
PROCESS BIOCHEMISTRY | 2019年 / 84卷
关键词：
Proteoglycan; Ganoderma lucidum spore powder; Hypoglycemic; Antibacterial; Antitumor; ULTRASOUND EXTRACTION; INDUCED APOPTOSIS; FRUITING BODIES; POLYSACCHARIDES; PROTEIN; CHITOSAN; NANOCOMPOSITES; OPTIMIZATION; FABRICATION; CELLS;
D O I：
10.1016/j.procbio.2019.05.025
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
A new natural proteoglycan from Ganoderma lucidum spore powder (GLSP) was obtained via solvent extraction and optimized using response surface methodology (RSM). The differences among the characteristics of the new proteoglycan from cracked (proteoglycan-C) and uncracked GLSP (proteoglycan-UC) were explored. The SDS-PAGE results showed the molecular weight of protein contained in proteoglycan-UC (55, 72, 95 kDa) was different to that proteoglycan-C (43, 95 kDa). The differences of these amino acids (Glu, Arg, Leu and Lys) content and monosaccharides (Fuc, Gal, Glc, Man and Gal-AC) content between proteoglycan-C and proteoglycan-UC were determined by HPLC/ion chromatography. The 2,2'-azino-bis (3-ethylbenzthiazoline-6-sulphonic acid) (ABTS) and 1,1-Diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activities of proteoglycan-C were higher than that proteoglycan-UC. The proteoglycan-UC exhibited stronger hypoglycemic and antibacterial effects against E. coli and S. aureus, and the proteoglycan-C and proteoglycan-UC showed antitumor effects with potential for therapeutic applications.
引用
收藏
页码：103 / 111
页数：9
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