A pea mutant (costata) expressing higher activity in thylakoid membrane-bound carbonic anhydrase alters PSII downregulation mechanisms

被引：5
作者：
Lazova, G. [1 ]
Naidenova, N. [2 ]
Ignatova, L. [3 ]
Stefanov, D. [1 ]
机构：
[1] Bulgarian Acad Sci, M Popov Inst Plant Physiol, BU-1113 Sofia, Bulgaria
[2] Bulgarian Acad Sci, Inst Genet, BU-1113 Sofia, Bulgaria
[3] Russian Acad Sci, Inst Basic Biol Problems, Pushchino 142290, Moskow Region, Russia
来源：
CELL BIOLOGY INTERNATIONAL | 2009年 / 33卷 / 08期
关键词：
Carbonic anhydrase; Pea; Costata mutant; Non-photochemical quenching; PSII downregulation; PHOTOSYNTHETIC ELECTRON-TRANSPORT; INACTIVE PHOTOSYSTEM-II; CHLOROPHYLL FLUORESCENCE; ABSORBENCY CHANGES; LIGHT; LEAVES; PHOTOPROTECTION; ETHOXYZOLAMIDE; CHLOROPLASTS; CAROTENOIDS;
D O I：
10.1016/j.cellbi.2009.04.003
中图分类号：
Q2 [细胞生物学];
学科分类号：
071009 ; 090102 ;
摘要：
The interaction between photosynthetic electron transport and the activities of the thylakoid associated carbonic anhydrase (tCA), estimated as combined tCA activity in pea plants (Pisum sativum L. Borek cv., WT) and mutant form (costata 2/125) that differ in chlorophyll content have been compared. Chlorophyll a fluorescence changes after the inhibition of tCA by ethoxyzolamide (EZ), estimating possible role of tCA in PSII downregulation were investigated. Costata expresses higher tCA activity and higher O-2 evolution in comparison to WT. Inhibition of tCA by EZ decreased effective PSII photochemistry that coincided with an enhancement in thermal dissipation, while maximal PSII quantum yield (F-v/F-m) did not significantly change. Ethoxyzolamide induced changes in fluorescence parameters that were more strongly expressed in costata 2/125. The results show that tCA is involved in the regulation of the proton gradient across thylakoid membranes and thus limits PSII downregulation. (C) 2009 International Federation for Cell Biology. Published by Elsevier Ltd. All rights reserved.
引用
收藏
页码：867 / 873
页数：7
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