Rutin-loaded liquid crystalline nanoparticles attenuate oxidative stress in bronchial epithelial cells: a PCR validation

被引：22
|
作者：
Mehta, Meenu [1 ,2 ]
Paudel, Keshav Raj [2 ,3 ]
Shukla, Shakti D. [4 ]
Shastri, Madhur D. [5 ]
Satija, Saurabh [6 ]
Singh, Sachin Kumar [6 ]
Gulati, Monica [6 ]
Dureja, Harish [7 ]
Zacconi, Flavia C. [8 ]
Hansbro, Philip M. [2 ,3 ]
Chellappan, Dinesh Kumar [9 ]
Dua, Kamal [1 ,2 ,4 ,10 ]
机构：
[1] Univ Technol Sydney, Grad Sch Hlth, Discipline Pharm, Sydney, NSW 2007, Australia
[2] Centenary Inst, Ctr Inflammat, Sydney, NSW 2050, Australia
[3] Univ Technol Sydney, Fac Sci, Sch Life Sci, Sydney, NSW 2007, Australia
[4] Univ Newcastle, Prior Res Ctr Hlth Lungs, Hunter Med Res Inst HMRI, Newcastle, NSW 2305, Australia
[5] Univ Tasmania, Coll Hlth & Med, Sch Hlth Sci, Launceston, Tas 7250, Australia
[6] Lovely Profess Univ, Sch Pharmaceut Sci, Phagwara 144411, Punjab, India
[7] Maharshi Dayanand Univ, Dept Pharmaceut Sci, Rohtak 124001, Haryana, India
[8] Pontificia Univ Catolica Chile, Fac Quim & Farm, Dept Quim Organ, Av Vicuna Mackenna 4860, Santiago 7820436, Chile
[9] Int Med Univ IMU, Sch Pharm, Dept Life Sci, Kuala Lumpur 57000, Malaysia
[10] Shoolini Univ, Sch Pharmaceut Sci, Solan 173229, Himachal Prades, India
来源：
FUTURE MEDICINAL CHEMISTRY | 2021年 / 13卷 / 06期
关键词：
antioxidant; inflammation; lipopolysaccharide; oxidative stress genes; rutin; PSEUDOMONAS-AERUGINOSA; PERITONEAL-MACROPHAGES; LUNG INFLAMMATION; NRF2; LPS; PROTECTION; TOXICITY; RATS;
D O I：
10.4155/fmc-2020-0297
中图分类号：
R914 [药物化学];
学科分类号：
100701 ;
摘要：
Aim: In the present study, the inhibitory potential of rutin-loaded liquid crystalline nanoparticles (LCNs) on oxidative stress was determined in human bronchial epithelial cells (BEAS-2B) by analysing the expression levels of different antioxidant (NADPH quinine oxidoreductase-1 (NQO1); gamma-glutamyl cysteine synthetase catalytic subunit (GCLC)) and pro-oxidant (NADPH oxidase (Nox)-4; Nox2B) genes. Results: Our findings revealed that the rutin-loaded LCNs inhibited the genes, namely Nox2B and Nox4, which caused oxidative stress. In addition, these nanoparticles demonstrated an upregulation in the expression of the antioxidant genes Gclc and Nqo-1 in a dose-dependent manner. Conclusion: The study indicates the promising potential of rutin-loaded LCNs as an effective treatment strategy in patients with high oxidant loads in various respiratory diseases.
引用
收藏
页码：543 / 549
页数：7
相关论文
共 50 条
  • [1] Rutin loaded liquid crystalline nanoparticles inhibit lipopolysaccharide induced oxidative stress and apoptosis in bronchial epithelial cells in vitro
    Paudel, Keshav Raj
    Wadhwa, Ridhima
    Mehta, Meenu
    Chellappan, Dinesh Kumar
    Hansbro, Philip M.
    Dua, Kamal
    TOXICOLOGY IN VITRO, 2020, 68
  • [2] Ameliorative effects of rutin and rutin-loaded chitosan nanoparticles on testicular oxidative stress and histological damage induced by cyclophosphamide in male rats
    AbdElrazek, Dina A.
    Hassan, Neven H.
    Ibrahim, Marwa A.
    Hassanen, Eman I.
    Farroh, Khaled Y.
    Abass, H. I.
    FOOD AND CHEMICAL TOXICOLOGY, 2024, 184
  • [3] Rutin-loaded polymeric nanorods alleviate nephrolithiasis by inhibiting inflammation and oxidative stress in vivo and in vitro
    Saha, Sarmistha
    Mishra, Abhijit
    FOOD & FUNCTION, 2022, 13 (06) : 3632 - 3648
  • [4] Economic and Productive Comparison of Rutin and Rutin-Loaded Chitosan Alginate Nanoparticles Against Lead-Induced Oxidative Stress in Cobb and Arbor Broiler Breeds
    Wahed, Noha M.
    Abomosallam, Mohamed
    Hendam, Basma M.
    Shouman, Zeinab
    Hashem, Nada M. A.
    Sakr, Shimaa A.
    BIOLOGICAL TRACE ELEMENT RESEARCH, 2024, 202 (10) : 4715 - 4734
  • [5] Rutin-loaded chitosan nanoparticles alleviated Freund's adjuvant induced rheumatoid arthritis via modulating oxidative stress and inflammatory parameters in Wistar rats
    Gravandi, Mohammad Mehdi
    Pourmanouchehri, Zahra
    Behbood, Leila
    Fakhri, Sajad
    Mohammadi-Noori, Ehsan
    Zhaleh, Mohsen
    Shirvani, Sahel
    Kiani, Amir
    Farzaei, Mohammad Hosein
    NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY, 2024, 397 (07) : 4771 - 4790
  • [6] Berberine-loaded engineered nanoparticles attenuate TGF-β-induced remodelling in human bronchial epithelial cells
    De Rubis, Gabriele
    Paudel, Keshav Raj
    Liu, Gang
    Agarwal, Vipul
    MacLoughlin, Ronan
    Pinto, Terezinha de Jesus Andreoli
    Singh, Sachin Kumar
    Adams, Jon
    Nammi, Srinivas
    Chellappan, Dinesh Kumar
    Oliver, Brian Gregory George
    Hansbro, Philip Michael
    Dua, Kamal
    TOXICOLOGY IN VITRO, 2023, 92
  • [7] Phytantriol-Based Berberine-Loaded Liquid Crystalline Nanoparticles Attenuate Inflammation and Oxidative Stress in Lipopolysaccharide-Induced RAW264.7 Macrophages
    Alnuqaydan, Abdullah M.
    Almutary, Abdulmajeed G.
    Azam, Mohd
    Manandhar, Bikash
    De Rubis, Gabriele
    Madheswaran, Thiagarajan
    Paudel, Keshav Raj
    Hansbro, Philip M.
    Chellappan, Dinesh Kumar
    Dua, Kamal
    NANOMATERIALS, 2022, 12 (23)
  • [8] Effects of Cyclosporine on Oxidative Stress in Human Bronchial Epithelial Cells
    Jeon, D. S.
    Ha, E. Y.
    Mun, K. C.
    TRANSPLANTATION PROCEEDINGS, 2012, 44 (04) : 988 - 990
  • [9] Oxidative stress and adaptive response of glutathione in bronchial epithelial cells
    Rahman, I
    MacNee, W
    CLINICAL AND EXPERIMENTAL ALLERGY, 2002, 32 (04): : 486 - 488
  • [10] Clothianidin induces DNA damage and oxidative stress in bronchial epithelial cells
    Atli Sekeroglu, Zulal
    Sekeroglu, Vedat
    Aydin, Birsen
    Kontas Yedier, Seval
    Ilkun, Emre
    ENVIRONMENTAL AND MOLECULAR MUTAGENESIS, 2020, 61 (06) : 647 - 655
12345