Monophenolase activity of strawberry polyphenol oxidase

被引：29
作者：
Espin, JC
Ochoa, M
Tudela, J
GarciaCanovas, F
机构：
[1] UNIV MURCIA,FAC BIOL,DEPT BIOQUIM & BIOL MOL A,GRP INVEST ENZIMOL,GENZ,E-30080 MURCIA,SPAIN
[2] UNIV NACL COMAHUE,FAC INGN,DEPT QUIM,RA-8336 VILLA REGINA,RIO NEGRO,ARGENTINA
来源：
PHYTOCHEMISTRY | 1997年 / 45卷 / 04期
关键词：
Fragaria x ananassa; Rosaceae; strawberry; 4-hydroxyanisole; 3-methyl-2-benzothiazolinone hydrazone; monophenolase; polyphenol oxidase;
D O I：
10.1016/S0031-9422(97)00052-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Strawberry polyphenol oxidase (PPO) was extracted and partially purified by using three sequential phase partitionings with Triton X-114 (TX-114) reported here for the first time. Monophenolase activity of strawberry PPO was reported for the first time. The enzyme fulfilled all the tests of the kinetic reaction mechanism previously proposed for PPOs from other sources. Therefore, this mechanism was also applicable to monophenolase activity of strawberry PPO. (C) 1997 Elsevier Science Ltd.
引用
收藏
页码：667 / 670
页数：4
相关论文
共 27 条
[1]   A KINETIC-STUDY OF THE MELANIZATION PATHWAY BETWEEN L-TYROSINE AND DOPACHROME [J].
CABANES, J ;
GARCIACANOVAS, F ;
LOZANO, JA ;
GARCIACARMONA, F .
BIOCHIMICA ET BIOPHYSICA ACTA, 1987, 923 (02) :187-195
[2]   Monophenolase activity of polyphenol oxidase from Blanquilla pear [J].
Espin, JC ;
Morales, M ;
Varon, R ;
Tudela, J ;
GarciaCanovas, F .
PHYTOCHEMISTRY, 1997, 44 (01) :17-22
[3]   A CONTINUOUS SPECTROPHOTOMETRIC METHOD FOR DETERMINING THE MONOPHENOLASE AND DIPHENOLASE ACTIVITIES OF APPLE POLYPHENOL OXIDASE [J].
ESPIN, JC ;
MORALES, M ;
VARON, R ;
TUDELA, J ;
GARCIACANOVAS, F .
ANALYTICAL BIOCHEMISTRY, 1995, 231 (01) :237-246
[4]   Continuous spectrophotometric method for determining monophenolase and diphenolase activities of pear polyphenoloxidase [J].
Espin, JC ;
Morales, M ;
Varon, R ;
Tudela, J ;
GarciaCanovas, F .
JOURNAL OF FOOD SCIENCE, 1996, 61 (06) :1177-+
[5]   MONOPHENOLASE ACTIVITY OF POLYPHENOL OXIDASE FROM VERDEDONCELLA APPLE [J].
ESPIN, JC ;
MORALES, M ;
VARON, R ;
TUDELA, J ;
GARCIACANOVAS, F .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1995, 43 (11) :2807-2812
[6]   HYDROPHOBIC ADSORPTION CHROMATOGRAPHY OF PEACH POLYPHENOL OXIDASE [J].
FLURKEY, WH ;
JEN, JJ .
JOURNAL OF FOOD SCIENCE, 1980, 45 (06) :1622-1624
[7]   PURIFICATION OF PEACH POLYPHENOL OXIDASE IN THE PRESENCE OF ADDED PROTEASE INHIBITORS [J].
FLURKEY, WH ;
JEN, JJ .
JOURNAL OF FOOD BIOCHEMISTRY, 1980, 4 (01) :29-41
[8]   KINETIC-STUDIES ON APPLE POLYPHENOL OXIDASE [J].
JANOVITZKLAPP, AH ;
RICHARD, FC ;
GOUPY, PM ;
NICOLAS, JJ .
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 1990, 38 (07) :1437-1441
[9]  
LAVOLLAY J, 1975, PHYSIOL VEG, V13, P667
[10]  
Matheis G., 1987, Chemie Mikrobiologie Technologie der Lebensmittel, V11, P5
123