Monophenolase activity of strawberry polyphenol oxidase

被引：29
作者：
Espin, JC
Ochoa, M
Tudela, J
GarciaCanovas, F
机构：
[1] UNIV MURCIA,FAC BIOL,DEPT BIOQUIM & BIOL MOL A,GRP INVEST ENZIMOL,GENZ,E-30080 MURCIA,SPAIN
[2] UNIV NACL COMAHUE,FAC INGN,DEPT QUIM,RA-8336 VILLA REGINA,RIO NEGRO,ARGENTINA
来源：
PHYTOCHEMISTRY | 1997年 / 45卷 / 04期
关键词：
Fragaria x ananassa; Rosaceae; strawberry; 4-hydroxyanisole; 3-methyl-2-benzothiazolinone hydrazone; monophenolase; polyphenol oxidase;
D O I：
10.1016/S0031-9422(97)00052-6
中图分类号：
Q5 [生物化学]; Q7 [分子生物学];
学科分类号：
071010 ; 081704 ;
摘要：
Strawberry polyphenol oxidase (PPO) was extracted and partially purified by using three sequential phase partitionings with Triton X-114 (TX-114) reported here for the first time. Monophenolase activity of strawberry PPO was reported for the first time. The enzyme fulfilled all the tests of the kinetic reaction mechanism previously proposed for PPOs from other sources. Therefore, this mechanism was also applicable to monophenolase activity of strawberry PPO. (C) 1997 Elsevier Science Ltd.
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页码：667 / 670
页数：4
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