Gene-wide histone acetylation at the yeast INO1 requires the transcriptional activator Ino2p

被引：7

作者：

Esposito, Michelle ^{[1
]}

Konarzewska, Paulina ^{[1
]}

Odeyale, Oluwafemi ^{[1
]}

Shen, Chang-Hui ^{[1
,2
]}

机构：

[1] CUNY Coll Staten Isl, Dept Biol, Staten Isl, NY 10314 USA

[2] CUNY, Inst Macromol Assemblies, Staten Isl, NY 10314 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2010年 / 391卷 / 02期

关键词：

INO1; INO2; Activator-dependent histone acetylation; Nucleosome density; Gene activation; Chromatin immunoprecipitation; PHOSPHOLIPID BIOSYNTHETIC GENES; SACCHAROMYCES-CEREVISIAE; CHROMATIN; PROMOTER; COMPLEX; RECRUITMENT; SWI/SNF; NUCLEOSOMES; DEACETYLASE; EXPRESSION;

D O I：

10.1016/j.bbrc.2009.12.063

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The relationship between histone acetylation and transcriptional activation at the yeast INO1 gene was addressed. INO1 encodes a key enzyme required for the de novo synthesis of phosphatidylinositol. Induction of INO1 resulted in acetylation of both histones H3 and H4 at the INO1 promoter and sequences farther downstream in the coding region, suggesting a gene-wide acetylation in response to transcriptional activation. Such chromatin remodeling activity requires the presence of transcriptional activator Ino2p. This indicates that histone acetylation is an activator-dependent event. Furthermore, the increase of histone acetylation is due to the increase of acetylation levels per nucleosome rather than the increase of nucleosome density. Therefore, these observations constitute evidence for the molecular mechanism of the correlation between histone acetylation and INO1 activity. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：1285 / 1290

页数：6

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