Fluorescent amplified-fragment length polymorphism analysis of the MRSA epidemic

被引:12
作者
Grady, R
O'Neill, G
Cookson, B
Stanley, J
机构
[1] Cent Publ Hlth Lab, Mol Biol Unit, London NW9 5HT, England
[2] Cent Publ Hlth Lab, Lab Hosp Infect, London NW9 5HT, England
关键词
fluorescent amplified-fragment length polymorphism; methicillin-resistant Staphylococcus aureus;
D O I
10.1111/j.1574-6968.2000.tb09131.x
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Genotypes and putative genetic relationships were characterised for epidemic methicillin-resistant Staphylococcus aureus (EMRSA) strains and isolates from England and Wales, using a high resolution DNA fingerprinting technique, fluorescent amplified-fragment length polymorphism (FAFLP). Each of the phage types of EMRSA had a distinct FAFLP profile. The technique revealed clusters of strains and isolates, and could distinguish isolates belonging to the same phage type. FAFLP provides a new approach to the epidemiological study and control of MRSA. (C) 2000 Federation of European Microbiological Societies. Published by Elsevier Science B.V. All rights reserved.
引用
收藏
页码:27 / 30
页数:4
相关论文
共 11 条
  • [1] Ayliffe GAJ, 1998, J HOSP INFECT, V39, P253, DOI 10.1016/S0195-6701(98)90293-6
  • [2] PULSED-FIELD GEL-ELECTROPHORESIS AS A REPLACEMENT FOR BACTERIOPHAGE-TYPING OF STAPHYLOCOCCUS-AUREUS
    BANNERMAN, TL
    HANCOCK, GA
    TENOVER, FC
    MILLER, JM
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1995, 33 (03) : 551 - 555
  • [3] BLAIR JE, 1961, B WORLD HEALTH ORGAN, V24, P771
  • [4] Inter-centre comparison of pulsed-field gel electrophoresis for the typing of methicillin-resistant Staphylococcus aureus
    Cookson, BD
    Aparicio, P
    Deplano, A
    Struelens, M
    Goering, R
    Marples, R
    [J]. JOURNAL OF MEDICAL MICROBIOLOGY, 1996, 44 (03) : 179 - 184
  • [5] Genotyping of epidemic methicillin-resistant Staphylococcus aureus phage type 15 isolates by fluorescent amplified fragment length polymorphism analysis
    Grady, R
    Desai, M
    O'Neill, G
    Cookson, B
    Stanley, J
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1999, 37 (10) : 3198 - 3203
  • [6] Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene
    Hookey, JV
    Richardson, JF
    Cookson, BD
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (04) : 1083 - 1089
  • [7] IANDOLO JJ, 1997, STAPHYLOCOCCI HUMAN, P39
  • [8] Page RDM, 1996, COMPUT APPL BIOSCI, V12, P357
  • [9] Sambrook J., 2002, MOL CLONING LAB MANU
  • [10] Assessment of resolution and intercenter reproducibility of results of genotyping Staphylococcus aureus by pulsed-field gel electrophoresis of SmaI macrorestriction fragments:: a multicenter study
    van Belkum, A
    van Leeuwen, W
    Kaufmann, ME
    Cookson, B
    Forey, F
    Etienne, J
    Goering, R
    Tenover, F
    Steward, C
    O'Brien, F
    Grubb, W
    Tassios, P
    Legakis, N
    Morvan, A
    El Solh, N
    de Ryck, R
    Struelens, M
    Salmenlinna, S
    Vuopio-Varkila, J
    Kooistra, M
    Talens, A
    Witte, W
    Verbrugh, H
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 1998, 36 (06) : 1653 - 1659