A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism

被引:24
作者
de Moliner, Fabio [1 ]
Knox, Kirsten [2 ]
Gordon, Doireann [1 ]
Lee, Martin [3 ]
Tipping, William J. [4 ,5 ]
Geddis, Ailsa [1 ,4 ]
Reinders, Anke [6 ]
Ward, John M. [6 ]
Oparka, Karl [2 ]
Vendrell, Marc [1 ]
机构
[1] Univ Edinburgh, Ctr Inflammat Res, Edinburgh, Midlothian, Scotland
[2] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh, Midlothian, Scotland
[3] Univ Edinburgh, Canc Res UK Edinburgh Ctr, Edinburgh, Midlothian, Scotland
[4] Univ Edinburgh, EaStCHEM Sch Chem, Edinburgh, Midlothian, Scotland
[5] Univ Strathclyde, Ctr Mol Nanometrol, Glasgow, Lanark, Scotland
[6] Univ Minnesota, Dept Plant & Microbial Biol, Minneapolis, MN 55455 USA
基金
英国生物技术与生命科学研究理事会; 英国医学研究理事会;
关键词
imaging; probes; stimulated Raman scattering; sugars; transport;
D O I
10.1002/anie.202016802
中图分类号
O6 [化学];
学科分类号
0703 ;
摘要
Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (approximate to 50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.
引用
收藏
页码:7637 / 7642
页数:6
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