A Palette of Minimally Tagged Sucrose Analogues for Real-Time Raman Imaging of Intracellular Plant Metabolism

被引：24

作者：

de Moliner, Fabio ^{[1
]}

Knox, Kirsten ^{[2
]}

Gordon, Doireann ^{[1
]}

Lee, Martin ^{[3
]}

Tipping, William J. ^{[4
,5
]}

Geddis, Ailsa ^{[1
,4
]}

Reinders, Anke ^{[6
]}

Ward, John M. ^{[6
]}

Oparka, Karl ^{[2
]}

Vendrell, Marc ^{[1
]}

机构：

[1] Univ Edinburgh, Ctr Inflammat Res, Edinburgh, Midlothian, Scotland

[2] Univ Edinburgh, Inst Mol Plant Sci, Edinburgh, Midlothian, Scotland

[3] Univ Edinburgh, Canc Res UK Edinburgh Ctr, Edinburgh, Midlothian, Scotland

[4] Univ Edinburgh, EaStCHEM Sch Chem, Edinburgh, Midlothian, Scotland

[5] Univ Strathclyde, Ctr Mol Nanometrol, Glasgow, Lanark, Scotland

[6] Univ Minnesota, Dept Plant & Microbial Biol, Minneapolis, MN 55455 USA

来源：

ANGEWANDTE CHEMIE-INTERNATIONAL EDITION | 2021年 / 60卷 / 14期

基金：

英国医学研究理事会; 英国生物技术与生命科学研究理事会;

关键词：

imaging; probes; stimulated Raman scattering; sugars; transport;

D O I：

10.1002/anie.202016802

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

Sucrose is the main saccharide used for long-distance transport in plants and plays an essential role in energy metabolism; however, there are no analogues for real-time imaging in live cells. We have optimised a synthetic approach to prepare sucrose analogues including very small (approximate to 50 Da or less) Raman tags in the fructose moiety. Spectroscopic analysis identified the alkyne-tagged compound 6 as a sucrose analogue recognised by endogenous transporters in live cells and with higher Raman intensity than other sucrose derivatives. Herein, we demonstrate the application of compound 6 as the first optical probe to visualise real-time uptake and intracellular localisation of sucrose in live plant cells using Raman microscopy.

引用

页码：7637 / 7642

页数：6

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