A Chemical Labeling Strategy for Proteomics under Nondenaturing Conditions

被引：6

作者：

Budin, Ghyslain ^{[1
]}

Dimala, Martin Moune ^{[2
]}

Lamour, Valerie ^{[2
,3
]}

Oudet, Pierre ^{[2
,3
]}

Mioskowski, Charles ^{[1
]}

Meunier, Stephane ^{[1
]}

Brino, Laurent ^{[2
]}

Wagner, Alain ^{[1
]}

机构：

[1] UMR 7199, Lab Funct Chemo Syst, F-67401 Illkirch Graffenstaden, France

[2] CNRS UdS, INSERM, Inst Genet & Mol & Cellular Biol, Lab Struct Biol & Genom,U964,UMR7104, Illkirch Graffenstaden, France

[3] Hautepierre Strasbourg Hosp, Biochem & Mol Biol Lab, Strasbourg, France

来源：

CHEMBIOCHEM | 2010年 / 11卷 / 01期

关键词：

activity-based probes; bio-orthogonal; click chemistry; fluorescent probes; nondenaturing conditions; BINDS COUMARIN DRUGS; DNA GYRASE; AFFINITY-CHROMATOGRAPHY; TERMINAL ALKYNES; PROTEIN; DISCOVERY; AZIDES; CYCLOADDITION; INHIBITORS; LIGATION;

D O I：

10.1002/cbic.200900641

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

(Figure Presented) Extra-mild nondenaturing protocol: Fishing for unaltered protein in a lysate is a prerequisite for identifying and analyzing the architecture of a protein complex. We have shown that a noncross-linked protein-ligand complex can resist bio-orthogonal Staudinger ligation. The resulting target-probe-label entity is compatible with nondenaturing protein analysis. © 2010 Wiley-VCH Verlag GmbH & Co. KGaA.

引用

页码：79 / 82

页数：4

共 30 条

[1] THE 43-KILODALTON N-TERMINAL FRAGMENT OF THE DNA GYRASE-B PROTEIN HYDROLYZES ATP AND BINDS COUMARIN DRUGS
ALI, JA
JACKSON, AP
HOWELLS, AJ
MAXWELL, A
[J]. BIOCHEMISTRY, 1993, 32 (10) : 2717 - 2724
[2] Can cell systems biology rescue drug discovery?
Butcher, EC
[J]. NATURE REVIEWS DRUG DISCOVERY, 2005, 4 (06) : 461 - 467
[3] Antimicrobial and DNA gyrase-inhibitory activities of novel clorobiocin derivatives produced by mutasynthesis
Galm, U
Heller, S
Shapiro, S
Page, M
Li, SM
Heide, L
[J]. ANTIMICROBIAL AGENTS AND CHEMOTHERAPY, 2004, 48 (04) : 1307 - 1312
[4] THE 24 KDA N-TERMINAL SUBDOMAIN OF THE DNA GYRASE-B PROTEIN BINDS COUMARIN DRUGS
GILBERT, EJ
MAXWELL, A
[J]. MOLECULAR MICROBIOLOGY, 1994, 12 (03) : 365 - 373
[5] An efficient proteomics method to identify the cellular targets of protein kinase inhibitors
Godl, K
Wissing, J
Kurtenbach, A
Habenberger, P
Blencke, S
Gutbrod, H
Salassidis, K
Stein-Gerlach, M
Missio, A
Cotten, M
Daub, H
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2003, 100 (26) : 15434 - 15439
[6] Guiffant Damien, 2007, Biotechnology Journal, V2, P68, DOI 10.1002/biot.200600223
[7] Affinity-based tagging of protein families with reversible inhibitors: A concept for functional proteomics
Hagenstein, MC
Mussgnug, JH
Lotte, K
Plessow, R
Brockhinke, A
Kruse, O
Sewald, N
[J]. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2003, 42 (45) : 5635 - 5638
[8] Directed immobilization of peptide ligands to accessible pore sites by conjugation with a placeholder molecule
Hahn, R
Berger, E
Pflegerl, K
Jungbauer, A
[J]. ANALYTICAL CHEMISTRY, 2003, 75 (03) : 543 - 548
[9] Incorporation of azides into recombinant proteins for chemoselective modification by the Staudinger ligation
Kiick, KL
Saxon, E
Tirrell, DA
Bertozzi, CR
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2002, 99 (01) : 19 - 24
[10] The nature of inhibition of DNA gyrase by the coumarins and the cyclothialidines revealed by X-ray crystallography
Lewis, RJ
Singh, OMP
Smith, CV
Skarzynski, T
Maxwell, A
Wonacott, AJ
Wigley, DB
[J]. EMBO JOURNAL, 1996, 15 (06) : 1412 - 1420

← 1 2 3 →