Angiotensin I-converting enzyme inhibitory peptides derived from wakame (Undaria pinnatifida) and their antihypertensive effect in spontaneously hypertensive rats

被引:219
作者
Sato, M
Hosokawa, T
Yamaguchi, T
Nakano, T
Muramoto, K
Kahara, T
Funayama, K
Kobayashi, A
Nakano, T
机构
[1] Tohoku Univ, Grad Sch Agr Sci, Aoba Ku, Sendai, Miyagi 9818555, Japan
[2] Riken Vitamin Co Ltd, Chiyoda Ku, Tokyo 1018370, Japan
关键词
wakame; Undaria pinnatifida; angiotensin I-converting enzyme; peptide; anti hypertension; spontaneously hypertensive rat; LC-MS; isolation; identification; digestive resistance;
D O I
10.1021/jf020482t
中图分类号
S [农业科学];
学科分类号
09 ;
摘要
Seven kinds of angiotensin I-converting enzyme (ACE) inhibitory peptides were isolated from the hydrolysates of wakame (Undaria pinnatifida) by Protease S "Amano" (from Bacillus stearothermophilus) by using three-step high-performance liquid chromatography (HPLC) on a reverse-phase column. These peptides were identified by amino acid composition analysis, sequence analysis, and liquid chromatography-mass spectrometry (LC-MS), as Val-Tyr (IC50 = 35.2 muM), Ile-Tyr (6.1 muM), Ala-Trp (18.8 muM), Phe-Tyr (42.3 muM), Val-Trp (3.3 muM), Ile-Trp (1.5 muM), and Leu-Trp (23.6 muM). These peptides have resistance against gastrointestinal proteases in vitro. Each peptide was determined to have an anti hypertensive effect after a single oral administration in spontaneously hypertensive rats (SHR). Among them, the blood pressure significantly decreased by Val-Tyr, Ile-Tyr, Phe-Tyr, and Ile-Trp in a dose of 1 mg/kg of body weight (BW). The present study showed that antihypertensive effect in the hydrolysates of wakame by Protease S "Amano" was attributed to these peptides.
引用
收藏
页码:6245 / 6252
页数:8
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