Development of a coaxial DCF-GRIN fiberoptic Raman probe for enhancing in vivo epithelial tissue Raman measurements

被引:3
作者
Heng, Howard Peng Sin [1 ]
Shu, Chi [1 ]
Zheng, Wei [1 ]
Huang, Zhiwei [1 ]
机构
[1] Natl Univ Singapore, Coll Design & Engn, Dept Biomed Engn, Opt Bioimaging Lab, Singapore 117576, Singapore
基金
英国医学研究理事会;
关键词
OPTICAL COHERENCE TOMOGRAPHY; SPECTROSCOPY; DIAGNOSIS;
D O I
10.1364/OL.474464
中图分类号
O43 [光学];
学科分类号
070207 ; 0803 ;
摘要
We report on the development of a novel, to the best of our knowledge, coaxial double-clad-fiber (DCF) and graded-index (GRIN) fiberoptic Raman probe for enhancing epithelial tissue Raman measurements in vivo. The ultrathin (140 mu m outer diameter) DCF-GRIN fiberoptic Raman probe is designed and fabricated with an efficient coaxial optical configuration, whereby a GRIN fiber is spliced onto the DCF to enhance both the excitation/collection efficiency and depth-resolved selectivity. We demonstrate that the DCF-GRIN Raman probe can be used to acquire high-quality in vivo Raman spectra from various oral tissues (e.g., buccal mucosa, labial mucosa, gingiva, mouth floor, palate, and tongue) covering both the fingerprint (800-1800 cm(-1)) and high-wavenumber (2800-3600 cm(-1)) regions within sub-seconds. The subtle biochemical differences between different epithelial tissues in the oral cavity can also be detected with high sensitivity, suggesting the potential of the DCF-GRIN fiberoptic Raman probe for in vivo diagnosis and characterization in epithelial tissue. (c) 2022 Optica Publishing Group
引用
收藏
页码:5989 / 5992
页数:4
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