Use of dot-immunobinding assay for visual detection of rinderpest antibodies in vaccinated cattle

被引:0
作者
Singh, G
Singh, I
机构
[1] Department of Veterinary Public Health and Epidemiology, Punjab Agricultural University
关键词
D O I
10.1007/BF02632321
中图分类号
S8 [畜牧、 动物医学、狩猎、蚕、蜂];
学科分类号
0905 ;
摘要
A dot-immunoblotting (DIB) assay was used to detect rinderpest antibodies in cattle vaccinated with Kabete 'O' strain vaccine, using purified rinderpest virus. Of 120 serum samples from vaccinated and non-vaccinated animals, rinderpest antibodies were detected in 80%, 88.4% and 91.6% of samples at 2, 3 and 4 weeks postvaccination respectively. All the serum samples from non-vaccinated animals were negative. The DIB results had a good correlation with those of the micro neutralisation test. The technique is simple, easy to perform and suitable for routine use in detecting rinderpest antibodies following vaccination.
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收藏
页码:73 / 76
页数:4
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