A direct RT-qPCR approach to test large numbers of individuals for SARS-CoV-2

被引：15

作者：

Maricic, Tomislav ^{[1
]}

Nickel, Olaf ^{[2
]}

Aximu-Petri, Ayinuer ^{[1
]}

Essel, Elena ^{[1
]}

Gansauge, Marie ^{[1
]}

Kanis, Philipp ^{[1
]}

Macak, Dominik ^{[1
]}

Richter, Julia ^{[1
]}

Riesenberg, Stephan ^{[1
]}

Bokelmann, Lukas ^{[1
]}

Zeberg, Hugo ^{[1
,3
]}

Meyer, Matthias ^{[1
]}

Borte, Stephan ^{[2
,4
,5
]}

Paeaebo, Svante ^{[1
]}

机构：

[1] Max Planck Inst Evolutionary Anthropol, Leipzig, Germany

[2] Hosp St Georg, Dept Lab Med, Leipzig, Germany

[3] Karolinska Inst, Dept Neurosci, Stockholm, Sweden

[4] Hosp St Georg Leipzig, Jeffrey Modell Diagnost & Res Ctr Primary Immunod, ImmunoDeficiencyCtr Leipzig IDCL, Leipzig, Germany

[5] Karolinska Univ Hosp Huddinge, Div Clin Immunol, Dept Lab Med, Karolinska Inst, Stockholm, Sweden

来源：

PLOS ONE | 2020年 / 15卷 / 12期

关键词：

D O I：

10.1371/journal.pone.0244824

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

SARS-CoV-2 causes substantial morbidity and mortality in elderly and immunocompromised individuals, particularly in retirement homes, where transmission from asymptomatic staff and visitors may introduce the infection. Here we present a cheap and fast screening method based on direct RT-qPCR to detect SARS-CoV-2 in single or pooled gargle lavages ("mouthwashes"). This method detects individuals with large viral loads (Ct <= 29) and we use it to test all staff at a nursing home daily over a period of three weeks in order to reduce the risk that the infection penetrates the facility. This or similar approaches can be implemented to protect hospitals, nursing homes and other institutions in this and future viral epidemics.

引用

页数：10

共 13 条

[1]

Baird PR, 2020, NEW YORKER

[2] Detection of 2019 novel coronavirus (2019-nCoV) by real-time RT-PCR (Publication with Expression of Concern) [J].

Corman, Victor M. ;

Landt, Olfert ;

Kaiser, Marco ;

Molenkamp, Richard ;

Meijer, Adam ;

Chu, Daniel K. W. ;

Bleicker, Tobias ;

Bruenink, Sebastian ;

Schneider, Julia ;

Schmidt, Marie Luisa ;

Mulders, Daphne G. J. C. ;

Haagmans, Bart L. ;

van der Veer, Bas ;

van den Brink, Sharon ;

Wijsman, Lisa ;

Goderski, Gabriel ;

Romette, Jean-Louis ;

Ellis, Joanna ;

Zambon, Maria ;

Peiris, Malik ;

Goossens, Herman ;

Reusken, Chantal ;

Koopmans, Marion P. G. ;

Drosten, Christian .

EUROSURVEILLANCE, 2020, 25 (03) :23-30

[3] Asymptomatic Transmission, the Achilles' Heel of Current Strategies to Control Covid-19 [J].

Gandhi, Monica ;

Yokoe, Deborah S. ;

Havlir, Diane V. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 382 (22) :2158-2160

[4]

He X, 2020, NAT MED, V26, DOI [10.1038/s41591-020-1016-z, 10.1038/s41591-020-0869-5]

[5]

Larremore DB, 2021, SCI ADV, V7, DOI [10.1126/sciadv.abd5393, 10.1101/2020.06.22.20136309]

[6] Pharynx gargle samples are suitable for SARS-CoV-2 diagnostic use and save personal protective equipment and swabs [J].

Malecki, Monika ;

Lusebrink, Jessica ;

Teves, Stefanie ;

Wendel, Andreas F. .

INFECTION CONTROL AND HOSPITAL EPIDEMIOLOGY, 2021, 42 (02) :248-249

[7] Epidemiology of Covid-19 in a Long-Term Care Facility in King County, Washington [J].

McMichael, Temet M. ;

Currie, Dustin W. ;

Clark, Shauna ;

Pogosjans, Sargis ;

Kay, Meagan ;

Schwartz, Noah G. ;

Lewis, James ;

Baer, Atar ;

Kawakami, Vance ;

Lukoff, Margaret D. ;

Ferro, Jessica ;

Brostrom-Smith, Claire ;

Rea, Thomas D. ;

Sayre, Michael R. ;

Riedo, Francis X. ;

Russell, Denny ;

Hiatt, Brian ;

Montgomery, Patricia ;

Rao, Agam K. ;

Chow, Eric J. ;

Tobolowsky, Farrell ;

Hughes, Michael J. ;

Bardossy, Ana C. ;

Oakley, Lisa P. ;

Jacobs, Jesica R. ;

Stone, Nimalie D. ;

Reddy, Sujan C. ;

Jernigan, John A. ;

Honein, Margaret A. ;

Clark, Thomas A. ;

Duchin, Jeffrey S. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 382 (21) :2005-2011

[8] Rethinking Covid-19 Test Sensitivity - A Strategy for Containment [J].

Mina, Michael J. ;

Parker, Roy ;

Larremore, Daniel B. .

NEW ENGLAND JOURNAL OF MEDICINE, 2020, 383 (22)

[9] Gargle lavage as a viable alternative to swab for detection of SARS-CoV-2 [J].

Mittal, Ankit ;

Gupta, Ankesh ;

Kumar, Shiv ;

Surjit, Milan ;

Singh, Binit ;

Soneja, Manish ;

Soni, Kapil Dev ;

Khan, Adil Rashid ;

Singh, Komal ;

Naik, Shivdas ;

Kumar, Arvind ;

Aggarwal, Richa ;

Nischal, Neeraj ;

Sinha, Sanjeev ;

Trikha, Anjan ;

Wig, Naveet .

INDIAN JOURNAL OF MEDICAL RESEARCH, 2020, 152 (01) :77-81

[10] Prevalence of Asymptomatic SARS-CoV-2 Infection A Narrative Review [J].

Oran, Daniel P. ;

Topol, Eric J. .

ANNALS OF INTERNAL MEDICINE, 2020, 173 (05) :362-+

← 1 2 →