Evanescent wave cavity ring-down spectroscopy based interfacial sensing of prostate-specific antigen

被引:7
作者
Chauhan, Anuj [1 ]
Dhenadhayalan, Namasivayam [1 ,2 ]
Lin, King-Chuen [1 ,2 ]
机构
[1] Natl Taiwan Univ, Dept Chem, Taipei 10617, Taiwan
[2] Acad Sinica, Inst Atom & Mol Sci, Taipei 10617, Taiwan
来源
SENSORS AND ACTUATORS B-CHEMICAL | 2021年 / 330卷
关键词
Evanescent wave cavity ring-down spectroscopy; Prostate-specific antigen; Gold nanoparticle; Biosensor; Biomarker; ABSORPTION-SPECTROSCOPY; BIOMARKER DETECTION; CRYSTAL VIOLET; ADSORPTION; PROBE; PSA; NANOPARTICLES; IMMUNOASSAY; KINETICS; RANGE;
D O I
10.1016/j.snb.2020.129284
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Evanescent wave cavity ring-down spectroscopy (EW-CRDS) is implemented as a sensitive probe for the detection of prostate-specific antigen (PSA) with a sub-femtomolar limit of detection (LOD). PSA, a glycoprotein, is an essential biomarker for scrutinizing prostate cancer. Gold nanoparticles (Au NPs) were synthesized to serve as a sensitive reporter which are strongly bound to the thiol-modified target DNA. While Au NPs/target DNA flowed via a microfluidic channel, the target DNA may hybridize with aptamer DNA immobilized on the silica surface. Thus, the adsorption kinetics of hybridization between the Au NPs/target and aptamer was measured with interfacial optical absorbance using Langmuir fit of adsorption equilibrium. The LOD of Au NPs/PSA sensor reaches 0.54 fM signifying tremendous detecting proficiency of EW-CRDS. The evanescent wave is highly sensitive for small changes in absorption of material leading to a significant variation of the ring-down time constant. Moreover, Au NPs possess inherent excellent surface plasmonic properties that made them a promising probe material for the interfacial sensing process. To assess the significance of probe material, 5-carboxytetramethylrhodamine was substituted as a reporter to determine sensing kinetics, showing LOD of 99.1 fM. EW-CRDS approach could be used as an efficient sensing technique for the examination of malignant diseases and many biological reactions.
引用
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页数:9
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