Transforming growth factor-β3 expression up-regulates on cleft palates induced by 2,3,7,8-tetrachlorodibenzo-p-dioxin in mice

被引：13

作者：

Gan, Li-qiang ^{[1
]}

Fu, Yue-xian ^{[1
]}

Liu, Xing ^{[1
]}

Qiu, Lin ^{[1
]}

Wu, Sheng-de ^{[1
]}

Tian, Xiao-fei ^{[1
]}

Liu, Yan ^{[1
]}

Wei, Guang-hui ^{[1
]}

机构：

[1] Chongqing Med Univ, Dept Pediat Surg, Chongqing Childrens Hosp, Chongqing 400014, Peoples R China

来源：

TOXICOLOGY AND INDUSTRIAL HEALTH | 2009年 / 25卷 / 07期

关键词：

2; 3; 7; 8-Tetrachlorodibenzo-p-dioxin; cleft palate; transforming growth factor-beta 3; mouse; RETINOIC ACID; SHELF FUSION; IN-VITRO; DIFFERENTIATION; TCDD; PALATOGENESIS; PATHOGENESIS; APOPTOSIS; FETUSES; DEATH;

D O I：

10.1177/0748233709345940

中图分类号：

R1 [预防医学、卫生学];

学科分类号：

1004 ; 120402 ;

摘要：

2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) has been shown to induce cleft palate, in which the molecular etiology of the defect is poorly characterized. Recently, transforming growth factor-beta 3 (TGF-beta 3) has been indicated to play an essential role in the development of palatal shelves. In this developmental toxicity study, we investigated the effects of 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD) on the expression of TGF-beta 3 in fetal mice. Pregnant C57BL/6 mice were exposed to corn oil or TCDD (32 mu g/kg/day 64 mu g/kg/day, per os) at embryonic day 10 (ED10), a drastic inhibition of palatal shelves was induced. By using RT-PCR (reverse transcription-polymerase chain reaction) and Western blot, the expressions of TGF-beta 3 was investigated. We found that the expression of TGF-beta 3 was gradually up-regulated in TCDD-treated group. These results suggest that cleft palate can be induced by TCDD exposure, the modification of TGF-beta 3 is related to its pathogenesis.

引用

页码：473 / 478

页数：6

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