Cryo-EM Model of the Bullet-Shaped Vesicular Stomatitis Virus

被引：195

作者：

Ge, Peng ^{[1
,2
,3
,4
]}

Tsao, Jun ^{[5
]}

Schein, Stan ^{[2
,6
]}

Green, Todd J. ^{[5
]}

Luo, Ming ^{[5
]}

Zhou, Z. Hong ^{[1
,2
,3
,4
]}

机构：

[1] Univ Calif Los Angeles, Dept Microbiol Immunol & Mol Genet, Los Angeles, CA 90095 USA

[2] Univ Calif Los Angeles, Calif NanoSyst Inst, Los Angeles, CA 90095 USA

[3] Baylor Coll Med, Struct Computat Biol & Mol Biophys Program, Houston, TX 77030 USA

[4] Univ Texas Med Sch Houston, Dept Pathol & Lab Med, Houston, TX 77030 USA

[5] Univ Alabama, Dept Microbiol, Birmingham, AL 35294 USA

[6] Univ Calif Los Angeles, Dept Psychol, Los Angeles, CA 90095 USA

来源：

SCIENCE | 2010年 / 327卷 / 5966期

关键词：

MATRIX PROTEIN; CRYSTAL-STRUCTURE; GLYCOPROTEIN-G; DOMAIN; NUCLEOPROTEIN; COMPLEX; CELLS; FORM;

D O I：

10.1126/science.1181766

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Vesicular stomatitis virus (VSV) is a bullet-shaped rhabdovirus and a model system of negative-strand RNA viruses. Through direct visualization by means of cryo-electron microscopy, we show that each virion contains two nested, left-handed helices: an outer helix of matrix protein M and an inner helix of nucleoprotein N and RNA. M has a hub domain with four contact sites that link to neighboring M and N subunits, providing rigidity by clamping adjacent turns of the nucleocapsid. Side-by-side interactions between neighboring N subunits are critical for the nucleocapsid to form a bullet shape, and structure-based mutagenesis results support this description. Together, our data suggest a mechanism of VSV assembly in which the nucleocapsid spirals from the tip to become the helical trunk, both subsequently framed and rigidified by the M layer.

引用

页码：689 / 693

页数：5

共 20 条

[1] THE ROLE OF VESICULAR STOMATITIS-VIRUS MATRIX PROTEIN IN INHIBITION OF HOST-DIRECTED GENE-EXPRESSION IS GENETICALLY SEPARABLE FROM ITS FUNCTION IN VIRUS ASSEMBLY [J].

BLACK, BL ;

RHODES, RB ;

MCKENZIE, M ;

LYLES, DS .

JOURNAL OF VIROLOGY, 1993, 67 (08) :4814-4821

[2] Role of residues 121 to 124 of vesicular stomatitis virus matrix protein in virus assembly and virus-host interaction [J].

Connor, JH ;

McKenzie, MO ;

Lyles, DS .

JOURNAL OF VIROLOGY, 2006, 80 (08) :3701-3711

[3] Crystal structure of the oligomerization domain of the phosphoprotein of vesicular stomatitis virus [J].

Ding, HT ;

Green, TJ ;

Lu, SY ;

Luo, M .

JOURNAL OF VIROLOGY, 2006, 80 (06) :2808-2814

[4] Crystal structure of vesicular stomatitis virus matrix protein [J].

Gaudier, M ;

Gaudin, Y ;

Knossow, M .

EMBO JOURNAL, 2002, 21 (12) :2886-2892

[5] AGGREGATION OF VSV M-PROTEIN IS REVERSIBLE AND MEDIATED BY NUCLEATION SITES - IMPLICATIONS FOR VIRAL ASSEMBLY [J].

GAUDIN, Y ;

BARGE, A ;

EBEL, C ;

RUIGROK, RWH .

VIROLOGY, 1995, 206 (01) :28-37

[6] Conformational flexibility and polymerization of vesicular stomatitis virus matrix protein [J].

Gaudin, Y ;

Sturgis, J ;

Doumith, M ;

Barge, A ;

Robert, B ;

Ruigrok, RWH .

JOURNAL OF MOLECULAR BIOLOGY, 1997, 274 (05) :816-825

[7] Rhabdovirus Matrix Protein Structures Reveal a Novel Mode of Self-Association [J].

Graham, Stephen C. ;

Assenberg, Rene ;

Delmas, Olivier ;

Verma, Anil ;

Gholami, Alireza ;

Talbi, Chiraz ;

Owens, Raymond J. ;

Stuart, David I. ;

Grimes, Jonathan M. ;

Bourhy, Herve .

PLOS PATHOGENS, 2008, 4 (12)

[8] Structure of the vesicular stomatitis virus nucleoprotein-RNA complex [J].

Green, Todd J. ;

Zhang, Xin ;

Wertz, Gail W. ;

Luo, Ming .

SCIENCE, 2006, 313 (5785) :357-360

[9] Structure of the vesicular stomatitis virus nucleocapsid in complex with the nucleocapsid-binding domain of the small polymerase cofactor, P [J].

Green, Todd J. ;

Luo, Ming .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2009, 106 (28) :11713-11718

[10] Vesicular stomatitis virus: re-inventing the bullet [J].

Lichty, BD ;

Power, AT ;

Stojdl, DF ;

Bell, JC .

TRENDS IN MOLECULAR MEDICINE, 2004, 10 (05) :210-216

← 1 2 →