Transcriptional profiling of mouse projection neurons with VECTORseq

被引：0

作者：

Cheung, Victoria ^{[1
,2
]}

Chung, Philip ^{[3
]}

Feinberg, Evan H. ^{[1
,4
]}

机构：

[1] Univ Calif San Francisco, Dept Anat, San Francisco, CA 94158 USA

[2] Univ Calif San Francisco, Tetrad Grad Program, San Francisco, CA 94158 USA

[3] Univ Washington, Dept Anesthesiol & Pain Med, Seattle, WA 98195 USA

[4] Univ Calif San Francisco, Kavli Inst Fundamental Neurosci, San Francisco, CA 94158 USA

来源：

STAR PROTOCOLS | 2022年 / 3卷 / 03期

基金：

美国国家卫生研究院;

关键词：

Bioinformatics; Cell isolation; Gene expression; Genomics; Neuroscience; RNAseq; Sequence analysis; Sequencing; Single cell;

D O I：

10.1016/j.xpro.2022.101625

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Existing techniques for transcriptional profiling of projection neurons could be applied to only one neuronal population per experiment. To increase throughput, we developed VECTORseq, which repurposes retrogradely infecting viruses to deliver multiplexable RNA barcodes, enabling projection anatomy to be read out in single-cell datasets. In this protocol, we describe the delivery of viral barcodes to mouse brain to label different projection neurons. We then detail single-cell or nuclei isolation for sequencing, followed by the analysis of single-cell sequencing data. For complete details on the use and execution of this protocol, please refer to Cheung et al. (2021).

引用

页数：23

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