Validity of a single antibody-based lateral flow immunoassay depending on graphene oxide for highly sensitive determination of E. coli O157:H7 in minced beef and river water

被引：62

作者：

Abdelazeem Hassan, Abdelrahim Hussein ^{[1
,2
,3
]}

Francisco Bergua, Jose ^{[1
,2
]}

Morales-Narvaez, Eden ^{[4
]}

Mekoci, Arben ^{[1
,2
,5
]}

机构：

[1] CSIC, Catalan Inst Nanosci & Nanotechnol ICN2, Nanobioelect & Biosensors Grp, Campus UAB, Barcelona 08193, Spain

[2] BIST, Campus UAB, Barcelona 08193, Spain

[3] Beni Suef Univ, Fac Vet Med, Dept Food Hyg & Control, Bani Suwayf 62511, Egypt

[4] Ctr Invest Opt AC, Biophoton Nanosensors Lab, Loma Bosque 115,Lomas Campestre Leon, Guanajuato 37150, Mexico

[5] ICREA, Pg Lluis Co 23, Barcelona 08010, Spain

来源：

FOOD CHEMISTRY | 2019年 / 297卷

关键词：

Graphene oxide; Fluorescent lateral flow; E. coli O157:H7; Food safety; Minced beef; Water quality; NONRADIATIVE ENERGY-TRANSFER; RAPID DETECTION; SAMPLES; STRIP; MILK;

D O I：

10.1016/j.foodchem.2019.124965

中图分类号：

O69 [应用化学];

学科分类号：

081704 ;

摘要：

Considering the health risks of E. coli O157:H7 presence in food and water, an affordable and highly sensitive detection method is crucial. Herein, we report the first use of a single antibody-based fluorescent lateral flow immunoassay (FLFIA) depending on non-radiative energy transfer between graphene oxide and quantum dots for determination of E. coli O157: H7 in beef and river water. FLFIA showed a high sensitivity rate thousand-fold better than the conventional lateral flow (LF). In inoculated minced beef and river water samples, the limits of detection were 178 and 133 CFU g(-1) or mL(-1), respectively. Besides, it presented a high selectivity in the presence of other possible interfering bacteria. The single antibody approach reduced the assay cost to 60% less than the conventional LF. Alongside, the results could be read by portable LF readers or smartphones. These advantages offer FLFIA as a promising technology for pathogen detection in food and water.

引用

页数：10

共 36 条

[1] Highly sensitive and rapid determination of Escherichia coli O157:H7 in minced beef and water using electrocatalytic gold nanoparticle tags [J].