The Chemical Characterization of Nigerian Propolis samples and Their Activity Against Trypanosoma brucei

被引：44
作者：
Omar, Ruwida [1 ]
Igoli, John O. [1 ,2 ]
Zhang, Tong [3 ]
Gray, Alexander I. [1 ]
Ebiloma, Godwin U. [4 ]
Clements, Carol J. [1 ]
Fearnley, James [5 ]
Ebel, RuAngeli Edrada [1 ]
Paget, Tim [6 ]
de Koning, Harry P. [4 ]
Watson, David G. [1 ]
机构：
[1] Univ Strathclyde, Strathclyde Inst Pharm & Biomed Sci, 161 Cathedral St, Glasgow G4 0RE, Lanark, Scotland
[2] Univ Agr, Dept Chem, Phytochem Res Grp, Makurdi, Nigeria
[3] Univ Glasgow, Inst Canc Sci, Wolfson Wohl Canc Res Ctr, Switchback Rd, Glasgow G61 1QH, Lanark, Scotland
[4] Univ Glasgow, Coll Med Vet & Life Sci, Inst Infect Immun & Inflammat, Glasgow G12 8TA, Lanark, Scotland
[5] BeeVital, Whitby YO22 5JR, N Yorkshire, England
[6] Univ Sunderland, Dept Pharm Hlth & Well Being, Wharncliffe St, Sunderland SR1 3SD, Tyne & Wear, England
来源：
SCIENTIFIC REPORTS | 2017年 / 7卷
关键词：
CROSS-RESISTANCE; MELARSOPROL; PENTAMIDINE; TRANSPORTER;
D O I：
10.1038/s41598-017-01038-2
中图分类号：
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号：
07 ; 0710 ; 09 ;
摘要：
Profiling of extracts from twelve propolis samples collected from eight regions in Nigeria was carried out using high performance liquid chromatography (LC) coupled with evaporative light scattering (ELSD), ultraviolet detection (UV) and mass spectrometry (MS), gas chromatography mass spectrometry (GC-MS) and nuclear magnetic resonance spectroscopy (NMR). Principal component analysis (PCA) of the processed LC-MS data demonstrated the varying chemical composition of the samples. Most of the samples were active against Trypanosoma b. brucei with the highest activity being in the samples from Southern Nigeria. The more active samples were fractionated in order to isolate the component(s) responsible for their activity using medium pressure liquid chromatography (MPLC). Three xanthones, 1,3,7-trihydroxy-2,8-di-(3-methylbut-2-enyl) xanthone, 1,3,7-trihydroxy-4,8-di-(3-methylbut-2-enyl) xanthone a previously undescribed xanthone and three triterpenes:ambonic acid, mangiferonic acid and a mixture of alpha-amyrin with mangiferonic acid (1:3) were isolated and characterised by NMR and LC-MS. These compounds all displayed strong inhibitory activity against T.b. brucei but none of them had higher activity than the crude extracts. Partial least squares (PLS) modelling of the anti-trypanosomal activity of the sample extracts using the LC-MS data indicated that high activity in the extracts, as judged from LCMS2 data, could be correlated to denticulatain isomers in the extracts.
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页数：10
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