Formation of cellular close-ended tunneling nanotubes through mechanical deformation

被引：26

作者：

Chang, Minhyeok ^{[1
,4
]}

Lee, O-Chul ^{[1
]}

Bu, Gayun ^{[1
]}

Oh, Jaeho ^{[1
]}

Yunn, Na-Oh ^{[2
]}

Ryu, Sung Ho ^{[3
]}

Kwon, Hyung-Bae ^{[4
]}

Kolomeisky, Anatoly B. ^{[5
]}

Shim, Sang-Hee ^{[6
]}

Doh, Junsang ^{[7
]}

Jeon, Jae-Hyung ^{[1
,8
]}

Lee, Jong-Bong ^{[1
,8
,9
]}

机构：

[1] Pohang Univ Sci & Technol POSTECH, Dept Phys, Pohang 37673, South Korea

[2] POSTECH Biotech Ctr, Pohang 37673, South Korea

[3] POSTECH, Dept Life Sci, Pohang 37673, South Korea

[4] Johns Hopkins Univ, Sch Med, Dept Neurosci, Baltimore, MD 21205 USA

[5] Rice Univ, Dept Chem, Houston, TX 77005 USA

[6] Korea Univ, Dept Chem, Seoul 02481, South Korea

[7] Seoul Natl Univ, Dept Mat Sci & Engn, Seoul 08826, South Korea

[8] Asia Pacific Ctr Theoret Phys APCTP, Pohang 37673, South Korea

[9] POSTECH, Sch Interdisciplinary Biosci & Bioengn, Pohang 37673, South Korea

来源：

SCIENCE ADVANCES | 2022年 / 8卷 / 13期

基金：

美国国家卫生研究院; 新加坡国家研究基金会;

关键词：

MEMBRANE NANOTUBES; ACTIN;

D O I：

10.1126/sciadv.abj3995

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Membrane nanotubes or tunneling nanotubes (TNTs) that connect cells have been recognized as a previously unidentified pathway for intercellular transport between distant cells. However, it is unknown how this delicate structure, which extends over tens of micrometers and remains robust for hours, is formed. Here, we found that a TNT develops from a double filopodial bridge (DFB) created by the physical contact of two filopodia through helical deformation of the DFB. The transition of a DFB to a close-ended TNT is most likely triggered by disruption of the adhesion of two filopodia by mechanical energy accumulated in a twisted DFB when one of the DFB ends is firmly attached through intercellular cadherin-cadherin interactions. These studies pinpoint the mechanistic questions about TNTs and elucidate a formation mechanism.

引用

页数：11

共 28 条

[1] Twirling of Actin by Myosins II and V Observed via Polarized TIRF in a Modified Gliding Assay [J].