Differential regulation of CXCL12 and PACAP mRNA expression after focal and global ischemia

被引:20
作者
Riek-Burchardt, Monika [2 ]
Kolodziej, Angela [1 ]
Henrich-Noack, Petra [2 ]
Reymann, Klaus G. [2 ]
Hoellt, Volker [1 ]
Stumm, Ralf [1 ,3 ]
机构
[1] Otto VonGuericke Univ Magdegurg, Inst Pharmakol & Toxikol, D-39120 Magdeburg, Germany
[2] Leibniz Inst Neurobiol, D-39118 Magdeburg, Germany
[3] Otto VonGuericke Univ Magdegurg, Res Grp Mol & Syst Neuropharmacol, CBBS, D-39120 Magdeburg, Germany
关键词
CXCL12; PACAP; CXCR4; SDF-1; Neurogenesis; Inflammation; Neuroprotection; Stroke; Hippocampus; Stem cell; Ischemia; CYCLASE-ACTIVATING POLYPEPTIDE; POSTNATAL DENTATE GYRUS; CENTRAL-NERVOUS-SYSTEM; MU-OPIOID RECEPTOR; CEREBRAL-ISCHEMIA; STEM-CELLS; ADULT BRAIN; HIPPOCAMPAL-NEURONS; NEURAL PROGENITORS; GRANULE CELLS;
D O I
10.1016/j.neuropharm.2009.07.032
中图分类号
Q189 [神经科学];
学科分类号
071006 ;
摘要
Pituitary adenylate cyclase activating peptide (PACAP) and the chemokine stromal cell-derived factor (SDF-1) have been implicated in neuroprotection, neurogenesis, and regeneration. Focal ischemia is associated with rapid upregulation of PACAP in perifocal neurons and delayed induction of SDF-1 in hypoxic/ischemic tissues, the latter process being involved in the recruitment of stem cells and inflammatory cells. Here, we studied mRNA patterns of PACAR SDF-1 and the cognate receptors PAC1 and CXCR4 by in situ hybridization in the rat hippocampus after transient global ischemia, a rat model for programmed death of CA1 pyramidal neurons. Cell death in CA1 was not associated with local induction of PACAP and SDF-1 expression or recruitment of CXCR4-expressing infiltrates. However, there was a transient, almost complete loss of SDF-1 expression in microvessels in all hippocampal regions. Granule cells transiently showed a decrease of SDF-1 and an increase of PACAP expression. While PAC1 mRNA was moderately decreased throughout the hippocampus, CXCR4 expression was selectively increased in the subgranular layer. We propose that altered PACAP and SDF-1 gene expression in granule cells plays a role in regulated neurogenesis after global ischemia. The finding that programmed neuronal death after global ischemia was not associated with SDF-1 upregulation or recruitment of CXCR4-expressing cells is in sharp contrast to SDF-1/CXCR4-mediated infiltration of infarct tissue after focal ischemia. Hence, the different modes of neuronal death after focal and global ischemia are associated with distinct SDF-1 and PACAP gene regulation patterns and distinct reorganization mechanisms. (C) 2009 Elsevier Ltd. All rights reserved.
引用
收藏
页码:199 / 207
页数:9
相关论文
共 40 条
  • [1] Impaired nerve regeneration and enhanced neuroinflarnmatory response in mice lacking pituitary adenylyl cyclase activating peptide
    Armstrong, B. D.
    Abad, C.
    Chhith, S.
    Cheling-Lau, G.
    Hajji, O. E.
    Noblita, H.
    Waschek, J. A.
    [J]. NEUROSCIENCE, 2008, 151 (01) : 63 - 73
  • [2] Highly regionalized distribution of stromal cell-derived factor-1/CXCL12 in adult rat brain:: constitutive expression in cholinergic, dopaminergic and vasopressinergic neurons
    Banisadr, G
    Skrzydelski, D
    Kitabgi, P
    Rostène, W
    Parsadaniantz, SM
    [J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2003, 18 (06) : 1593 - 1606
  • [3] Expression of SDF-1 and CXCR4 during reorganization of the postnatal dentate gyrus
    Berger, Omri
    Li, Guangnan
    Han, Szu-Min
    Paredes, Mercedes
    Pleasure, Samuel J.
    [J]. DEVELOPMENTAL NEUROSCIENCE, 2007, 29 (1-2) : 48 - 58
  • [4] The chemokine stromal cell-derived factor-1 regulates GABAergic inputs to neural progenitors in the postnatal dentate gyrus
    Bhattacharyya, Bula J.
    Banisadr, Ghazal
    Jung, Hosung
    Ren, Dongjun
    Cronshaw, Darran G.
    Zou, Yongrui
    Miller, Richard J.
    [J]. JOURNAL OF NEUROSCIENCE, 2008, 28 (26) : 6720 - 6730
  • [5] Homing to hypoxia: HIF-1 as a mediator of progenitor cell recruitment to injured tissue
    Ceradini, DJ
    Gurtner, GC
    [J]. TRENDS IN CARDIOVASCULAR MEDICINE, 2005, 15 (02) : 57 - 63
  • [6] Ischemia-induced neuronal apoptosis
    Choi, DW
    [J]. CURRENT OPINION IN NEUROBIOLOGY, 1996, 6 (05) : 667 - 672
  • [7] Nitric Oxide Donor Up-Regulation of SDF1/CXCR4 and Ang1/Tie2 Promotes Neuroblast Cell Migration After Stroke
    Cui, Xu
    Chen, Jieli
    Zacharek, Alex
    Roberts, Cynthia
    Yang, Yuping
    Chopp, Michael
    [J]. JOURNAL OF NEUROSCIENCE RESEARCH, 2009, 87 (01) : 86 - 95
  • [8] DIRNAGL U, 1993, NEUROL RES, V15, P128
  • [9] The neuropeptide pituitary adenylate cyclase-activating polypeptide exerts anti-apoptotic and differentiating effects during neurogenesis:: Focus on cerebellar granule neurones and embryonic stem cells
    Falluel-Morel, A.
    Chafai, M.
    Vaudry, D.
    Basille, M.
    Cazillis, M.
    Aubert, N.
    Louiset, E.
    deJouffrey, S.
    Le Bigot, J. F.
    Fournier, A.
    Gressens, P.
    Rostene, W.
    Gressens, P.
    Rostene, W.
    Vaudry, H.
    Gonzalez, B. J.
    [J]. JOURNAL OF NEUROENDOCRINOLOGY, 2007, 19 (05) : 321 - 327
  • [10] Pituitary adenylate cyclase-activating peptide in the rat central nervous system: An immunohistochemical and in situ hybridization study
    Hannibal, J
    [J]. JOURNAL OF COMPARATIVE NEUROLOGY, 2002, 453 (04) : 389 - 417