Droplet Digital PCR for Quantitative Detection of Escherichia Coli

被引：0
|
作者：
Yang, Jiayi [1 ]
Fu, Boqiang [1 ]
Niu, Chunyan [1 ]
Wang, Jing [1 ]
机构：
[1] Natl Inst Metrol, Div Med & Biol Measurement, Beijing, Peoples R China
来源：
INTERNATIONAL SYMPOSIUM ON THE FRONTIERS OF BIOTECHNOLOGY AND BIOENGINEERING (FBB 2019) | 2019年 / 2110卷
基金：
国家重点研发计划;
关键词：
Escherichia Coli; Droplet Digital PCR; Quantification; Detection;
D O I：
10.1063/1.5110837
中图分类号：
Q81 [生物工程学（生物技术）]; Q93 [微生物学];
学科分类号：
071005 ; 0836 ; 090102 ; 100705 ;
摘要：
The bacterium, Escherichia coli, associates with both people health and water sanitation. It is widely treated as the indicator of faecal contamination in drinking water. Droplet digital PCR (dd-PCR) is an absolutely quantification method that can be used in Escherichia coli cell detection. Here, we have established a detection method based on droplet digital PCR for Escherichia coli (CICC10389), which shows good linearity and specificity within its detection range. Moreover, it also displays good linear correlation with the method based on real time quantification PCR. It would help to elevate detection efficiency for Escherichia coli cell in drinking water.
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页数：5
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