Effects of type I Diacylglycerol O-acyltransferase (DGAT1) genes on soybean (Glycine max L.) seed composition

被引：22

作者：

Torabi, Sepideh ^{[1
]}

Sukumaran, Arjun ^{[2
,3
]}

Dhaubhadel, Sangeeta ^{[2
,3
]}

Johnson, Sarah E. ^{[4
]}

LaFayette, Peter ^{[4
]}

Parrott, Wayne A. ^{[4
,5
]}

Rajcan, Istvan ^{[1
]}

Eskandari, Milad ^{[1
]}

机构：

[1] Univ Guelph, Dept Plant Agr, Guelph, ON, Canada

[2] Agr & Agri Food Canada, London Res & Dev Ctr, London, ON, Canada

[3] Univ Western Ontario, Dept Biol, London, ON, Canada

[4] Univ Georgia, Ctr Appl Genet Technol, Athens, GA 30602 USA

[5] Univ Georgia, Dept Crop & Soil Sci, Athens, GA 30602 USA

来源：

SCIENTIFIC REPORTS | 2021年 / 11卷 / 01期

关键词：

OIL CONTENT; TRIACYLGLYCEROL BIOSYNTHESIS; ARABIDOPSIS-THALIANA; BRASSICA-NAPUS; OVER-EXPRESSION; INCREASES OIL; FATTY-ACIDS; CLONING; IDENTIFICATION; PROTEIN;

D O I：

10.1038/s41598-021-82131-5

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

Type I Diacylglycerol acyltransferase (DGAT1) catalyzes the final step of the biosynthesis process of triacylglycerol (TAG), the major storage lipids in plant seeds, through the esterification of diacylglycerol (DAG). To characterize the function of DGAT1 genes on the accumulation of oil and other seed composition traits in soybean, transgenic lines were generated via trans-acting siRNA technology, in which three DGAT1 genes (Glyma.13G106100, Glyma.09G065300, and Glyma.17G053300) were downregulated. The simultaneous downregulation of the three isoforms in transgenic lines was found to be associated with the reduction of seed oil concentrations by up to 18 mg/g (8.3%), which was correlated with increases in seed protein concentration up to 42 mg/g (11%). Additionally, the downregulations also influenced the fatty acid compositions in the seeds of transgenic lines through increasing the level of oleic acid, up to 121 mg/g (47.3%). The results of this study illustrate the importance of DGAT1 genes in determining the seed compositions in soybean through the development of new potential technology for manipulating seed quality in soybean to meet the demands for its various food and industrial applications.

引用

页数：14

共 21 条

[21] Fine mapping of sterile genes in soybean (Glycine max [L.] Merril.) based on cross populations between multi-elite parent and sterile lines ms1 and ms6
Jiang, Zhenfeng
Song, Jiazhuo
Du, Yanwei
Hao, Hongmei
Cheng, Jiyao
Zhao, Caitong
Zhang, Xianfeng
Wang, Wanpeng
Li, Jianan
Zheng, Yuhong
Yang, Chunyan
Li, Wenbin
PLANT BREEDING, 2024, 143 (03) : 267 - 275

← 1 2 3 →