Safety and Functional Aspects of Preselected Enterococci for Probiotic Use in Iberian Dry-Fermented Sausages

被引:29
作者
Ruiz-Moyano, Santiago [1 ]
Martin, Alberto [1 ]
Jose Benito, Maria [1 ]
Aranda, Emilio [1 ]
Casquete, Rocio [1 ]
de Guia Cordoba, Maria [1 ]
机构
[1] Univ Extremadura, Escuela Ingn Agr, E-06071 Badajoz, Spain
关键词
Enterococcus faecium SE906; Iberian dry-fermented sausages; probiotic; LACTIC-ACID BACTERIA; ANTIBIOTIC-RESISTANCE; BIOGENIC-AMINES; VIRULENCE DETERMINANTS; STARTER CULTURES; FAECIUM STRAINS; MEAT-PRODUCTS; CACO-2; CELLS; IN-VIVO; IDENTIFICATION;
D O I
10.1111/j.1750-3841.2009.01290.x
中图分类号
TS2 [食品工业];
学科分类号
0832 ;
摘要
The purpose of this study was to investigate enterococci for potential probiotic use in Iberian dry-fermented sausages. A total of 15 strains isolated from Iberian dry- fermented sausages, human feces, and pig feces were evaluated for their safety and functional characteristics including biogenic amine (BA) production, antibiotic susceptibility, hemolysis, virulence determinants, cell adhesion, and antimicrobial activity against foodborne pathogens. The strain Enterococcus faecium SE906 was able to establish itself on the intestinal epithelium, inhibiting such pathogenic bacteria as Listeria monocytogenes in vitro. This strain was also considered safe to be used for its low aminogenic potential, and its antibiotic resistance pattern and virulence determinants, being identified as a potential probiotic meat starter culture suitable for manufacture of dry-fermented Iberian sausages.
引用
收藏
页码:M398 / M404
页数:7
相关论文
共 53 条
[1]   Recovery of Lactobacillus rhamnosus GG from human colonic biopsies [J].
Alander, M ;
Korpela, R ;
Saxelin, M ;
VilpponenSalmela, T ;
MattilaSandholm, T ;
vonWright, A .
LETTERS IN APPLIED MICROBIOLOGY, 1997, 24 (05) :361-364
[2]   Selection criteria for lactic acid bacteria to be used as functional starter cultures in dry sausage production: An update [J].
Ammor, Mohammed Salim ;
Mayo, Baltasar .
MEAT SCIENCE, 2007, 76 (01) :138-146
[3]   Control of Listeria monocytogenes in model sausages by enterocin AS-48 [J].
Ananou, S ;
Garriga, M ;
Hugas, M ;
Maqueda, M ;
Martínez-Bueno, M ;
Gálvez, A ;
Valdivia, E .
INTERNATIONAL JOURNAL OF FOOD MICROBIOLOGY, 2005, 103 (02) :179-190
[4]  
[Anonymous], 2001, OP SCI COMM AN NUTR
[5]   Application of enterocins as biopreservatives against Listeria innocua in meat products [J].
Aymerich, T ;
Garriga, M ;
Ylla, J ;
Vallier, J ;
Monfort, JM ;
Hugas, M .
JOURNAL OF FOOD PROTECTION, 2000, 63 (06) :721-726
[6]   Biochemical and genetic characterization of enterocin A from Enterococcus faecium, a new antilisterial bacteriocin in the pediocin family of bacteriocins [J].
Aymerich, T ;
Holo, H ;
Havarstein, LS ;
Hugas, M ;
Garriga, M ;
Nes, IF .
APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 1996, 62 (05) :1676-1682
[7]  
Ben Omar N, 2004, SYST APPL MICROBIOL, V27, P118, DOI 10.1078/0723-2020-00248
[8]   Characterization and selection of autochthonous lactic acid bacteria isolated from traditional Iberian dry-fermented salchichon and chorizo sausages [J].
Benito, M. J. ;
Martin, A. ;
Aranda, E. ;
Perez-Nevado, F. ;
Ruiz-Moyano, S. ;
Cordoba, M. G. .
JOURNAL OF FOOD SCIENCE, 2007, 72 (06) :M193-M201
[9]   Rapid differentiation of lactic acid bacteria from autochthonous fermentation of Iberian dry-fermented sausages [J].
Benito, Maria J. ;
Serradilla, Manuel J. ;
Ruiz-Moyano, Santiago ;
Martin, Alberto ;
Perez-Nevado, Francisco ;
Cordoba, Maria G. .
MEAT SCIENCE, 2008, 80 (03) :656-661
[10]   Adhesion studies for probiotics: need for validation and refinement [J].
Blum, S ;
Reniero, R ;
Schiffrin, EJ ;
Crittenden, R ;
Mattila-Sandholm, T ;
Ouwehand, AC ;
Salminen, S ;
von Wright, A ;
Saarela, M ;
Saxelin, M ;
Collins, K ;
Morelli, L .
TRENDS IN FOOD SCIENCE & TECHNOLOGY, 1999, 10 (12) :405-410