Prevalence of Porphyromonas gingivalis in relation to periodontal status assessed by real-time PCR

被引：43

作者：

Kawada, M ^{[1
]}

Yoshida, A ^{[1
]}

Suzuki, N ^{[1
]}

Nakano, Y ^{[1
]}

Saito, T ^{[1
]}

Oho, T ^{[1
]}

Koga, T ^{[1
]}

机构：

[1] Kyushu Univ, Fac Dent Sci, Dept Prevent Dent, Higashi Ku, Fukuoka 8128582, Japan

来源：

ORAL MICROBIOLOGY AND IMMUNOLOGY | 2004年 / 19卷 / 05期

关键词：

periodontitis; pocket depth; Porphyromonas gingivalis; real-time polymerase chain reaction; TaqMan assay;

D O I：

10.1111/j.1399-302X.2004.00154.x

中图分类号：

R78 [口腔科学];

学科分类号：

1003 ;

摘要：

Many studies have examined the presence of Porphyromonas gingivalis in periodontal pockets. However, monitoring the number of bacterial cells is difficult. In this study, we performed quantitative analyses of P. gingivalis to clarify the relationship between the numbers of this organism and periodontal status. Using the TaqMan real-time PCR system, we found a significant positive correlation (P < 0.0001) between the number of P. gingivalis and pocket depth. The slope of the regression line indicated that for every 1-mm increase in pocket depth, the number of P. gingivalis increased 10- fold. There was also a significant reduction (P < 0.01) in the numbers of P. gingivalis before and after treatment. These results suggest that the absolute and relative numbers of P. gingivalis are closely associated with periodontal status, and that quantitative analysis of this organism is important for the evaluation of periodontal therapy.

引用

页码：289 / 292

页数：4

共 25 条

[1]

[Anonymous], OR HLTH SURV BAS MET

[2] Comparison of microbial cultivation and a commercial PCR based method for detection of periodontopathogenic species in subgingival plaque samples [J].

Eick, S ;

Pfister, W .

JOURNAL OF CLINICAL PERIODONTOLOGY, 2002, 29 (07) :638-644

[3]

Fredricks DN, 1999, CLIN INFECT DIS, V29, P475, DOI 10.1086/598618

[4] PCR PRIMERS AND PROBES FOR THE 16S RIBOSOMAL-RNA GENE OF MOST SPECIES OF PATHOGENIC BACTERIA, INCLUDING BACTERIA FOUND IN CEREBROSPINAL-FLUID [J].

GREISEN, K ;

LOEFFELHOLZ, M ;

PUROHIT, A ;

LEONG, D .

JOURNAL OF CLINICAL MICROBIOLOGY, 1994, 32 (02) :335-351

[5] Clinical and microbiological features of subjects with adult periodontitis who responded poorly to scaling and root planing [J].

Haffajee, AD ;

Cugini, MA ;

Dibart, S ;

Smith, C ;

Kent, RL ;

Socransky, SS .

JOURNAL OF CLINICAL PERIODONTOLOGY, 1997, 24 (10) :767-776

[6]

Herzberg M C, 1998, Ann Periodontol, V3, P151, DOI 10.1902/annals.1998.3.1.151

[7] A rapid DNA probe method for detection of Porphyromonas gingivalis and Actinobacillus actinomycetemcomitans [J].

Komiya, A ;

Kato, T ;

Nakagawa, T ;

Saito, A ;

Takahashi, J ;

Yamada, S ;

Okuda, K .

JOURNAL OF PERIODONTOLOGY, 2000, 71 (05) :760-767

[8] COMPARISON OF VARIOUS DETECTION METHODS FOR PERIODONTOPATHIC BACTERIA - CAN CULTURE BE CONSIDERED THE PRIMARY REFERENCE-STANDARD [J].

LOESCHE, WJ ;

LOPATIN, DE ;

STOLL, J ;

VANPOPERIN, N ;

HUJOEL, PP .

JOURNAL OF CLINICAL MICROBIOLOGY, 1992, 30 (02) :418-426

[9] Persistence patterns of Porphyromonas gingivalis, Prevotella intermedia/nigrescens, and Actinobacillus actinomycetemcomitans after mechanical therapy of periodontal disease [J].

Mombelli, A ;

Schmid, B ;

Rutar, A ;

Lang, NP .

JOURNAL OF PERIODONTOLOGY, 2000, 71 (01) :14-21

[10] Correlation between oral malodor and periodontal bacteria [J].

Nakano, Y ;

Yoshimura, M ;

Koga, T .

MICROBES AND INFECTION, 2002, 4 (06) :679-683

← 1 2 3 →