Candida parapsilosis Colony Morphotype Forecasts Biofilm Formation of Clinical Isolates

被引:11
作者
Gomez-Molero, Emilia [1 ,2 ]
De-la-Pinta, Iker [3 ]
Fernandez-Pereira, Jordan [2 ]
Gross, Uwe [1 ]
Weig, Michael [1 ]
Quindos, Guillermo [3 ]
de Groot, Piet W. J. [2 ]
Bader, Oliver [1 ]
机构
[1] Univ Med Ctr Gottingen, Inst Med Microbiol, Kreuzbergring 57, D-37075 Gottingen, Germany
[2] Univ Castilla La Mancha, Reg Ctr Biomed Res, Castilla La Mancha Sci & Technol Pk, Albacete 02008, Spain
[3] Univ Pais Vasco UPV EHU, Sch Med & Nursing, Dept Immunol Microbiol & Parasitol, Bilbao 48940, Spain
关键词
Candida parapsilosis; biofilm; colony morphology; drug susceptibility;
D O I
10.3390/jof7010033
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
Candida parapsilosis is a frequent cause of fungal bloodstream infections, especially in critically ill neonates or immunocompromised patients. Due to the formation of biofilms, the use of indwelling catheters and other medical devices increases the risk of infection and complicates treatment, as cells embedded in biofilms display reduced drug susceptibility. Therefore, biofilm formation may be a significant clinical parameter, guiding downstream therapeutic choices. Here, we phenotypically characterized 120 selected isolates out of a prospective collection of 215 clinical C. parapsilosis isolates, determining biofilm formation, major emerging colony morphotype, and antifungal drug susceptibility of the isolates and their biofilms. In our isolate set, increased biofilm formation capacity was independent of body site of isolation and not predictable using standard or modified European Committee on Antimicrobial Susceptibility Testing (EUCAST) drug susceptibility testing protocols. In contrast, biofilm formation was strongly correlated with the appearance of non-smooth colony morphotypes and invasiveness into agar plates. Our data suggest that the observation of non-smooth colony morphotypes in cultures of C. parapsilosis may help as an indicator to consider the initiation of anti-biofilm-active therapy, such as the switch from azole- to echinocandin- or polyene-based strategies, especially in case of infections by potent biofilm-forming strains.
引用
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页码:1 / 12
页数:12
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