SPR Imaging for High Throughput, Label-Free Interaction Analysis

被引：36

作者：

Lausted, Christopher ^{[1
]}

Hu, Zhiyuan ^{[1
]}

Hood, Leroy ^{[1
]}

Campbell, Charles T. ^{[2
]}

机构：

[1] Inst Syst Biol, Seattle, WA 98103 USA

[2] Univ Washington, Dept Chem, Seattle, WA 98195 USA

来源：

COMBINATORIAL CHEMISTRY & HIGH THROUGHPUT SCREENING | 2009年 / 12卷 / 08期

基金：

美国国家科学基金会; 美国国家卫生研究院;

关键词：

SPR; surface plasmon resonance imaging; surface plasmon resonance microscopy; microarrays; bioaffinity; antibody screening; kinetics; binding constants; protein arrays; DNA arrays; SURFACE-PLASMON RESONANCE; SELF-ASSEMBLED MONOLAYERS; PROTEIN-DNA INTERACTIONS; CONTAINING ALKYLTHIOLATE MONOLAYERS; ULTRATHIN ORGANIC FILMS; MODIFIED GOLD SURFACES; REAL-TIME ANALYSIS; BIOMOLECULAR INTERACTIONS; RNA MICROARRAYS; OLIGONUCLEOTIDE SYNTHESIZER;

D O I：

10.2174/138620709789104933

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Surface plasmon resonance (SPR) sensors have proven themselves over the last 20 years to be an effective method to study biomolecular binding and kinetics without the use of labeling. More recently, the approach has been adapted to high throughput use with the imaging of SPR-active microarrays. This is an excellent tool for monitoring microarray binding in real-time where the microarray probes and targets can include a wide range of molecules. DNA, RNA, antibodies, enzymes, and a range of other proteins have been arrayed and quantitatively analyzed.

引用

页码：741 / 751

页数：11

共 116 条

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