Molecular mapping of male-sterility loci ms2 and ms9 in soybean

Markers linked to male-sterile, female-fertile loci on the soybean [Glycine mar (L.) Merr.] molecular map would facilitate early identification of male-sterile plants. The objectives were to verify the chromosome location of the ms2 (A00-63) mutation, to determine the allelism and the chromosome location of the suspected ms2 [ms? (A00-39)] mutation, and to determine the chromosome location of the ms9 (T359) mutation. Simple sequence repeat (SSR) markers were used to molecularly map the male-sterile, female-fertile loci reported in this study. Segregating F-2 populations were developed from crosses of ms?ms? (A00-39) x 'Minsoy' (PI 278901), ms2ms2 (A00-63) x Minsoy, and Minsoy x Ms9ms9 (T359H). The ms? (A00-39) locus was positioned on MLG O at 9.9 centiMorgans (cM) distance from the marker Sat_190. The ms2 (A00-63) locus was positioned on molecular linkage group (MLG) O between markers Sat_190 and ScaaOO1, with a distance of 6.9 and 9.0 cM, respectively. The ms9 locus was located on MLG N between markers Satt521 and Satt237, with a distance of 8.5 and 16.2 cM, respectively. Classical allelism tests confirmed that mutant ms? (A00-39) was allelic to ms2 (A00-63). The A00-39 mutant line was assigned Genetic Type Collection number T375H and gene symbol Ms2ms2 (Ames 2). Thus Genetic Type T360H, previously identified at Ames, Iowa, becomes Ms2ms2 (Ames 1).