Purification of a trypsin inhibitor from sweet potato in an aqueous two phase system

被引：9

作者：

Jaw, Kuen-Shan

Chou, Lin Hung

Chang, Shiao-Ming

Duan, Kow-Jen ^{[1
]}

机构：

[1] Tatung Univ, Dept Bioengn, Taipei 104, Taiwan

[2] No Taiwan Inst Sci & Technol, Dept Chem Engn, Taipei 112, Taiwan

来源：

BIOTECHNOLOGY LETTERS | 2007年 / 29卷 / 01期

关键词：

aqueous two-phase system; purification; sweet potato; trypsin inhibitor;

D O I：

10.1007/s10529-006-9210-8

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Trypsin inhibitor from sweet potato was extracted and purified in a single step using an aqueous two-phase system of polyethylene glycol 6000 (11% w/v), phosphate (16.5% w/v), KCl (9% w/v) and at pH 6. Purity of the trypsin inhibitor was enhanced 3.7-fold, and the recovery was 95%. The purified trypsin inhibitor showed one visible band, and the molecular size was 23 kDa by SDS-PAGE.

引用

页码：137 / 140

页数：4

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