Signal-Transducing Adaptor Protein-2 Regulates Stromal Cell-Derived Factor-1α-Induced Chemotaxis in T Cells

被引：31
作者：
Sekine, Yuichi
Ikeda, Osamu
Tsuji, Satoshi
Yamamoto, Chikako
Muromoto, Ryuta
Nanbo, Asuka
Oritani, Kenji [2 ]
Yoshimura, Akihiko [3 ]
Matsuda, Tadashi [1 ]
机构：
[1] Hokkaido Univ, Dept Immunol, Grad Sch Pharmaceut Sci, Kita Ku, Sapporo, Hokkaido 0600812, Japan
[2] Osaka Univ, Dept Hematol & Oncol, Grad Sch Med, Osaka, Japan
[3] Keio Univ, Sch Med, Dept Microbiol & Immunol, Shinjuku Ku, Tokyo, Japan
来源：
JOURNAL OF IMMUNOLOGY | 2009年 / 183卷 / 12期
关键词：
LYMPHOCYTE CHEMOATTRACTANT; ACTIVATION; ADHESION; CHEMOKINES; KINASE; RAC; RHO; RECEPTOR; GTPASES; CXCR4;
D O I：
10.4049/jimmunol.0902096
中图分类号：
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号：
100102 ;
摘要：
Signal-transducing adaptor protein-2 (STAP-2) is a recently identified adaptor protein that contains pleckstrin and Src homology 2-like domains, as well as a YXXQ motif in its C-terminal region. Our previous studies revealed that STAP-2 regulates integrin-mediated T cell adhesion. In the present study, we find that STAP-2 expression affects Jurkat T cell migration after stromal cell-derived factor-1 alpha (SDF-1 alpha)-treatment. Furthermore, STAP-2-deficient T cells exhibit reduced cell migration after SDF-1 alpha-treatment. Importantly, overexpression of STAP-2 in Jurkat T cells induces activation of small guanine triphosphatases, such as Rac1 and Cdc42. Regarding the mechanism for this effect, we found that STAP-2 associates with Vav1, the guanine-nucleotide exchanging factor for Rac1, and enhances downstream Vav1/Rac1 signaling. These results reveal a novel STAP-2-mediated mechanism for the regulation of SDF-1 alpha-induced chemotaxis of T cells via activation of Vav1/Rac1 signaling. The Journal of Immunology, 2009, 183: 7966-7974.
引用
收藏
页码：7966 / 7974
页数：9
相关论文
共 40 条
[11]   Vav1 and Rac control chemokine-promoted T lymphocyte adhesion mediated by the integrin α4β1 [J].
García-Bernal, D ;
Wright, N ;
Sotillo-Mallo, E ;
Nombela-Arrieta, C ;
Stein, JV ;
Bustelo, XR ;
Teixidó, J .
MOLECULAR BIOLOGY OF THE CELL, 2005, 16 (07) :3223-3235
[12]   The interaction between Cdc42 and WASP is required for SDF-1-induced T-lymphocyte chemotaxis [J].
Haddad, E ;
Zugaza, JL ;
Louache, F ;
Debili, N ;
Crouin, C ;
Schwarz, K ;
Fischer, A ;
Vainchenker, W ;
Bertoglio, J .
BLOOD, 2001, 97 (01) :33-38
[13]   STAP-2 negatively regulates both canonical and noncanonical NF-κB activation induced by Epstein-Barr virus-derived latent membrane protein 1 [J].
Ikeda, Osamu ;
Sekine, Yuichi ;
Yasui, Teruhito ;
Oritani, Kenji ;
Sugiyma, Kenji ;
Muromoto, Ryuta ;
Ohbayashi, Norihiko ;
Yoshimura, Akihiko ;
Matsuda, Tadashi .
MOLECULAR AND CELLULAR BIOLOGY, 2008, 28 (16) :5027-5042
[14]   Distinct role of ZAP-70 and Src homology 2 domain-containing leukocyte protein of 76 kDa in the prolonged activation of extracellular signal-regulated protein kinase by the stromal cell-derived factor-1α/CXCL12 chemokine [J].
Kremer, KN ;
Humphreys, TD ;
Kumar, A ;
Qian, NX ;
Hedin, KE .
JOURNAL OF IMMUNOLOGY, 2003, 171 (01) :360-367
[15]   CXCR4 physically associates with the T cell receptor to signal in T cells [J].
Kumar, Ashok ;
Hurnphreys, Troy D. ;
Kremer, Kimberly N. ;
Bramati, Patricia S. ;
Bradfield, Lavone ;
Edgar, Contessa E. ;
Hedin, Karen E. .
IMMUNITY, 2006, 25 (02) :213-224
[16]   Role of Rho in chemoattractant-activated leukocyte adhesion through integrins [J].
Laudanna, C ;
Campbell, JJ ;
Butcher, EC .
SCIENCE, 1996, 271 (5251) :981-983
[17]   Chemokines: immunology's high impact factors [J].
Mackay, CR .
NATURE IMMUNOLOGY, 2001, 2 (02) :95-101
[18]   Cross-talk between transforming growth factor-β and estrogen receptor signaling through Smad3 [J].
Matsuda, T ;
Yamamoto, T ;
Muraguchi, A ;
Saatcioglu, F .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2001, 276 (46) :42908-42914
[19]   STAP-2/BKS, an adaptor/docking protein, modulates STAT3 activation in acute-phase response through its YXXQ motif [J].
Minoguchi, M ;
Minoguchi, S ;
Aki, D ;
Joo, A ;
Yamamoto, T ;
Yumioka, T ;
Matsuda, T ;
Yoshimura, A .
JOURNAL OF BIOLOGICAL CHEMISTRY, 2003, 278 (13) :11182-11189
[20]   A novel adaptor-like protein which is a substrate for the non-receptor tyrosine kinase, BRK [J].
Mitchell, PJ ;
Sara, EA ;
Crompton, MR .
ONCOGENE, 2000, 19 (37) :4273-4282
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