PCR-based methods for identification of Enterococcus species

被引：21

作者：

Drahovská, H

Kocíncová, D

Seman, M

Turna, J

机构：

[1] Comenius Univ, Fac Sci, Dept Mol Biol, Bratislava 84215, Slovakia

[2] Comenius Univ, Fac Sci, Inst Cellular Biol, Bratislava 84215, Slovakia

来源：

FOLIA MICROBIOLOGICA | 2002年 / 47卷 / 06期

关键词：

D O I：

10.1007/BF02818665

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Two DNA-based techniques were used for species identification of enterococci. PvuII digestion of the genus-specific PCR product yielded four different restriction profiles among 20 enterococcal species; one of them was species-specific for E. faecium. In the second case, 32 reference strains belonging to 20 enterococcal species were divided to 12 groups by amplification of internal transcribed spacer of rRNA operon. Interspecies and some intraspecies profile variability was determined. Both methods gave similar results.

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页码：649 / 653

页数：5

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