A point mutation in the glutamate-gated chloride channel of Plutella xylostella is associated with resistance to abamectin

被引:71
作者
Wang, X. [1 ]
Wang, R. [1 ,2 ]
Yang, Y. [1 ]
Wu, S. [1 ]
O'Reilly, A. O. [3 ]
Wu, Y. [1 ]
机构
[1] Nanjing Agr Univ, Coll Plant Protect, Nanjing 210095, Jiangsu, Peoples R China
[2] Beijing Acad Agr & Forestry Sci, Inst Plant & Environm Protect, Beijing, Peoples R China
[3] Liverpool John Moores Univ, Sch Nat Sci & Psychol, Liverpool L3 5UX, Merseyside, England
基金
中国国家自然科学基金;
关键词
resistance; abamectin; glutamate-gated chloride channel; target site mutation; molecular docking; GENE SUPERFAMILY; DIAMONDBACK MOTH; LEPIDOPTERA; IVERMECTIN; HELIX; POPULATIONS; ACID;
D O I
10.1111/imb.12204
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The diamondback moth, Plutella xylostella, is a global pest of cruciferous vegetables. Abamectin resistance in a field population of P. xylostella was introgressed into the susceptible Roth strain. The resulting introgression strain Roth-Abm showed 11000-fold resistance to abamectin compared with Roth. An A309V substitution at the N-terminus of the third transmembrane helix (M3) of the glutamate-gated chloride channel of P. xylostella (PxGluCl) was identified in Roth-Abm. The frequency of the V309 allele of PxGluCl was 94.7% in Roth-Abm, whereas no such allele was detected in Roth. A subpopulation of Roth-Abm was kept without abamectin selection for 20 generations to produce a revertant strain, Roth-Abm-D. Abamectin resistance in Roth-Abm-D declined to 1150-fold compared with Roth, with the V309 allele frequency decreased to 9.6%. After treatment of the Roth-Abm-D strain with 80 mg/l abamectin the V309 allele frequency in the survivors increased to 55%. This demonstrates that the A309V mutation in PxGluCl is strongly associated with a 10-fold increase in abamectin resistance in Roth-Abm relative to Roth-Abm-D. Homology modelling and automated ligand docking results suggest that the A309V substitution allosterically modifies the abamectin-binding site, as opposed to directly eliminating a key binding contact. Other resistance mechanisms to abamectin in Roth-Abm are discussed besides the A309V mutation of PxGluCl.
引用
收藏
页码:116 / 125
页数:10
相关论文
共 44 条
[1]   X-ray structures of GluCl in apo states reveal a gating mechanism of Cys-loop receptors [J].
Althoff, Thorsten ;
Hibbs, Ryan E. ;
Banerjee, Surajit ;
Gouaux, Eric .
NATURE, 2014, 512 (7514) :333-+
[2]   STRUCTURAL BASIS OF AMINO-ACID ALPHA-HELIX PROPENSITY [J].
BLABER, M ;
ZHANG, XJ ;
MATTHEWS, BW .
SCIENCE, 1993, 260 (5114) :1637-1640
[3]   Cross-resistance and Inheritance of Resistance to Emamectin Benzoate in Spodoptera exigua (Lepidoptera: Noctuidae) [J].
Che, Wunan ;
Huang, Jianlei ;
Guan, Fang ;
Wu, Yidong ;
Yang, Yihua .
JOURNAL OF ECONOMIC ENTOMOLOGY, 2015, 108 (04) :2015-2020
[4]   RESISTANCE TO AVERMECTINS - EXTENT, MECHANISMS, AND MANAGEMENT IMPLICATIONS [J].
CLARK, JM ;
SCOTT, JG ;
CAMPOS, F ;
BLOOMQUIST, JR .
ANNUAL REVIEW OF ENTOMOLOGY, 1995, 40 :1-30
[5]   Inhibitory glutamate receptor channels [J].
Cleland, TA .
MOLECULAR NEUROBIOLOGY, 1996, 13 (02) :97-136
[6]   CLONING OF AN AVERMECTIN-SENSITIVE GLUTAMATE-GATED CHLORIDE CHANNEL FROM CAENORHABDITIS-ELEGANS [J].
CULLY, DF ;
VASSILATIS, DK ;
LIU, KK ;
PARESS, PS ;
VANDERPLOEG, LHT ;
SCHAEFFER, JM ;
ARENA, JP .
NATURE, 1994, 371 (6499) :707-711
[7]   Identification of a Drosophila melanogaster glutamate-gated chloride channel sensitive to the antiparasitic agent avermectin [J].
Cully, DF ;
Paress, PS ;
Liu, KK ;
Schaeffer, JM ;
Arena, JP .
JOURNAL OF BIOLOGICAL CHEMISTRY, 1996, 271 (33) :20187-20191
[8]   The genetics of ivermectin resistance in Caenorhabditis elegans [J].
Dent, JA ;
Smith, MM ;
Vassilatis, DK ;
Avery, L .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 2000, 97 (06) :2674-2679
[9]   The cys-loop ligand-gated ion channel gene family of Tetranychus urticae: Implications for acaricide toxicology and a novel mutation associated with abamectin resistance [J].
Dermauw, W. ;
Ilias, A. ;
Riga, M. ;
Tsagkarakou, A. ;
Grbic, M. ;
Tirry, L. ;
Van Leeuwen, T. ;
Vontas, J. .
INSECT BIOCHEMISTRY AND MOLECULAR BIOLOGY, 2012, 42 (07) :455-465
[10]   Exon 3 Splicing and Mutagenesis Identify Residues Influencing Cell Surface Density of Heterologously Expressed Silkworm (Bombyx mori) Glutamate-Gated Chloride Channels [J].
Furutani, Shogo ;
Ihara, Makoto ;
Nishino, Yuri ;
Akamatsu, Miki ;
Jones, Andrew K. ;
Sattelle, David B. ;
Matsuda, Kazuhiko .
MOLECULAR PHARMACOLOGY, 2014, 86 (06) :686-695