Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain

被引：40
作者：
Li, Xiaowei [1 ,2 ]
Tzeng, Stephany Y. [1 ,3 ]
Liu, Xiaoyan [4 ]
Tammia, Markus [1 ,2 ]
Cheng, Yu-Hao [1 ]
Rolfe, Andrew [5 ]
Sun, Dong [5 ]
Zhang, Ning [4 ]
Green, Jordan J. [1 ,2 ,3 ,6 ]
Wen, Xuejun [7 ,8 ]
Mao, Hai-Quan [1 ,2 ]
机构：
[1] Johns Hopkins Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USA
[2] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA
[3] Johns Hopkins Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA
[4] Virginia Commonwealth Univ, Sch Engn, Dept Biomed Engn, Richmond, VA 23284 USA
[5] Virginia Commonwealth Univ, Dept Neurosurg, Med Coll Virginia, Richmond, VA 23298 USA
[6] Johns Hopkins Univ, Sch Med, Dept Neurosurg, Baltimore, MD 21205 USA
[7] Virginia Commonwealth Univ, Sch Engn, Dept Chem & Life Sci Engn, Richmond, VA 23284 USA
[8] Virginia Commonwealth Univ, 601 West Main St, Richmond, VA 23284 USA
来源：
BIOMATERIALS | 2016年 / 84卷
基金：
美国国家科学基金会;
关键词：
Nanoparticles; Transfection; Neurogenin-2; Neuronal differentiation; Traumatic brain injury; GENE DELIVERY; PRECURSOR CELLS; GROWTH-FACTOR; DNA DELIVERY; TRANSFECTION; SURVIVAL; ADULT; EXPRESSION; GENERATION; VECTORS;
D O I：
10.1016/j.biomaterials.2016.01.037
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：157 / 166
页数：10
相关论文
共 39 条
  • [11] Rapid and Efficient Generation of Functional Motor Neurons From Human Pluripotent Stem Cells Using Gene Delivered Transcription Factor Codes
    Hester, Mark E.
    Murtha, Matthew J.
    Song, SungWon
    Rao, Meghan
    Miranda, Carlos J.
    Meyer, Kathrin
    Tian, Jinbin
    Boulting, Gabriella
    Schaffer, David V.
    Zhu, Michael X.
    Pfaff, Samuel L.
    Gage, Fred H.
    Kaspar, Brian K.
    [J]. MOLECULAR THERAPY, 2011, 19 (10) : 1905 - 1912
  • [12] Transplantation of human embryonic stem cell-derived neural precursor cells and enriched environment after cortical stroke in rats: cell survival and functional recovery
    Hicks, Anna U.
    Lappalainen, Riikka S.
    Narkilahti, Susanna
    Suuronen, Riitta
    Corbett, Dale
    Sivenius, Juhani
    Hovatta, Outi
    Jolkkonen, Jukka
    [J]. EUROPEAN JOURNAL OF NEUROSCIENCE, 2009, 29 (03) : 562 - 574
  • [13] Survival of transplanted human neural stem cell line (ReNcell VM) into the rat brain with and without immunosuppression
    Hovakimyan, M.
    Mueller, J.
    Wree, A.
    Ortinau, S.
    Rolfs, A.
    Schmitt, O.
    [J]. ANNALS OF ANATOMY-ANATOMISCHER ANZEIGER, 2012, 194 (05) : 429 - 435
  • [14] Genetic modification of neural stem cells
    Jandial, Rahul
    Singec, Ilyas
    Ames, Christopher P.
    Snyder, Evan Y.
    [J]. MOLECULAR THERAPY, 2008, 16 (03) : 450 - 457
  • [15] Traumatic brain injury and recovery mechanisms: peptide modulation of periventricular neurogenic regions by the choroid plexus-CSF nexus
    Johanson, Conrad
    Stopa, Edward
    Baird, Andrew
    Sharma, Hari
    [J]. JOURNAL OF NEURAL TRANSMISSION, 2011, 118 (01) : 115 - 133
  • [16] NEW PROCEDURE FOR DNA TRANSFECTION WITH POLYCATION AND DIMETHYLSULFOXIDE
    KAWAI, S
    NISHIZAWA, M
    [J]. MOLECULAR AND CELLULAR BIOLOGY, 1984, 4 (06) : 1172 - 1174
  • [17] Li X., 2013, METHODS COMPOSITIONS
  • [18] Short Laminin Peptide for Improved Neural Stem Cell Growth
    Li, Xiaowei
    Liu, Xiaoyan
    Josey, Benjamin
    Chou, C. James
    Tan, Yu
    Zhang, Ning
    Wen, Xuejun
    [J]. STEM CELLS TRANSLATIONAL MEDICINE, 2014, 3 (05) : 662 - 670
  • [19] Engineering an in situ crosslinkable hydrogel for enhanced remyelination
    Li, Xiaowei
    Liu, Xiaoyan
    Cui, Lin
    Brunson, Christopher
    Zhao, Wen
    Bhat, Narayan R.
    Zhang, Ning
    Wen, Xuejun
    [J]. FASEB JOURNAL, 2013, 27 (03) : 1127 - 1136
  • [20] Improve the viability of transplanted neural cells with appropriate sized neurospheres coated with mesenchymal stem cells
    Li, Xiaowei
    Liu, Xiaoyan
    Tan, Yu
    Van Tran
    Zhang, Ning
    Wen, Xuejun
    [J]. MEDICAL HYPOTHESES, 2012, 79 (02) : 274 - 277
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