Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain

被引：40
作者：
Li, Xiaowei [1 ,2 ]
Tzeng, Stephany Y. [1 ,3 ]
Liu, Xiaoyan [4 ]
Tammia, Markus [1 ,2 ]
Cheng, Yu-Hao [1 ]
Rolfe, Andrew [5 ]
Sun, Dong [5 ]
Zhang, Ning [4 ]
Green, Jordan J. [1 ,2 ,3 ,6 ]
Wen, Xuejun [7 ,8 ]
Mao, Hai-Quan [1 ,2 ]
机构：
[1] Johns Hopkins Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USA
[2] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA
[3] Johns Hopkins Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA
[4] Virginia Commonwealth Univ, Sch Engn, Dept Biomed Engn, Richmond, VA 23284 USA
[5] Virginia Commonwealth Univ, Dept Neurosurg, Med Coll Virginia, Richmond, VA 23298 USA
[6] Johns Hopkins Univ, Sch Med, Dept Neurosurg, Baltimore, MD 21205 USA
[7] Virginia Commonwealth Univ, Sch Engn, Dept Chem & Life Sci Engn, Richmond, VA 23284 USA
[8] Virginia Commonwealth Univ, 601 West Main St, Richmond, VA 23284 USA
来源：
BIOMATERIALS | 2016年 / 84卷
基金：
美国国家科学基金会;
关键词：
Nanoparticles; Transfection; Neurogenin-2; Neuronal differentiation; Traumatic brain injury; GENE DELIVERY; PRECURSOR CELLS; GROWTH-FACTOR; DNA DELIVERY; TRANSFECTION; SURVIVAL; ADULT; EXPRESSION; GENERATION; VECTORS;
D O I：
10.1016/j.biomaterials.2016.01.037
中图分类号：
R318 [生物医学工程];
学科分类号：
0831 ;
摘要：
Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy. (C) 2016 Elsevier Ltd. All rights reserved.
引用
收藏
页码：157 / 166
页数：10
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