Nanoparticle-mediated transcriptional modification enhances neuronal differentiation of human neural stem cells following transplantation in rat brain

被引：40

作者：

Li, Xiaowei ^{[1
,2
]}

Tzeng, Stephany Y. ^{[1
,3
]}

Liu, Xiaoyan ^{[4
]}

Tammia, Markus ^{[1
,2
]}

Cheng, Yu-Hao ^{[1
]}

Rolfe, Andrew ^{[5
]}

Sun, Dong ^{[5
]}

Zhang, Ning ^{[4
]}

Green, Jordan J. ^{[1
,2
,3
,6
]}

Wen, Xuejun ^{[7
,8
]}

Mao, Hai-Quan ^{[1
,2
]}

机构：

[1] Johns Hopkins Sch Med, Translat Tissue Engn Ctr, Baltimore, MD 21287 USA

[2] Johns Hopkins Univ, Dept Mat Sci & Engn, Baltimore, MD 21218 USA

[3] Johns Hopkins Sch Med, Dept Biomed Engn, Baltimore, MD 21205 USA

[4] Virginia Commonwealth Univ, Sch Engn, Dept Biomed Engn, Richmond, VA 23284 USA

[5] Virginia Commonwealth Univ, Dept Neurosurg, Med Coll Virginia, Richmond, VA 23298 USA

[6] Johns Hopkins Univ, Sch Med, Dept Neurosurg, Baltimore, MD 21205 USA

[7] Virginia Commonwealth Univ, Sch Engn, Dept Chem & Life Sci Engn, Richmond, VA 23284 USA

[8] Virginia Commonwealth Univ, 601 West Main St, Richmond, VA 23284 USA

来源：

BIOMATERIALS | 2016年 / 84卷

基金：

美国国家科学基金会;

关键词：

Nanoparticles; Transfection; Neurogenin-2; Neuronal differentiation; Traumatic brain injury; GENE DELIVERY; PRECURSOR CELLS; GROWTH-FACTOR; DNA DELIVERY; TRANSFECTION; SURVIVAL; ADULT; EXPRESSION; GENERATION; VECTORS;

D O I：

10.1016/j.biomaterials.2016.01.037

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 ;

摘要：

Strategies to enhance survival and direct the differentiation of stem cells in vivo following transplantation in tissue repair site are critical to realizing the potential of stem cell-based therapies. Here we demonstrated an effective approach to promote neuronal differentiation and maturation of human fetal tissue derived neural stem cells (hNSCs) in a brain lesion site of a rat traumatic brain injury model using biodegradable nanoparticle-mediated transfection method to deliver key transcriptional factor neurogenin-2 to hNSCs when transplanted with a tailored hyaluronic acid (HA) hydrogel, generating larger number of more mature neurons engrafted to the host brain tissue than non-transfected cells. The nanoparticle-mediated transcription activation method together with an HA hydrogel delivery matrix provides a translatable approach for stem cell-based regenerative therapy. (C) 2016 Elsevier Ltd. All rights reserved.

引用

页码：157 / 166

页数：10

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