Applicability of TOF-SIMS for the Assessment of Lipid Composition of Cell Membrane Structures

被引：2

作者：

Gulin, A. A. ^{[1
,2
]}

Pavlyukov, M. S. ^{[3
]}

Gusev, S. A. ^{[4
]}

Malakhova, Yu. N. ^{[5
,6
]}

Buzin, A. I. ^{[7
]}

Chvalun, S. N. ^{[5
,7
]}

Aldarov, K. G. ^{[4
]}

Klinov, D. V. ^{[4
]}

Gularyan, S. K. ^{[1
]}

Nadtochenko, V. A. ^{[1
,2
]}

机构：

[1] Russian Acad Sci, Semenov Inst Chem Phys, Ul Kosygina 4, Moscow 119991, Russia

[2] Lomonosov Moscow State Univ, Dept Chem, Leninskiye Gory 1,Bldg 3, Moscow 119991, Russia

[3] Russian Acad Sci, Shemyakin Ovchinnikov Inst Bioorgan Chem, Ul Miklukho Maklaya 16-10, Moscow 117997, Russia

[4] Fed Med & Biol Agcy SRI Phys Chem Med, Ul Malaya Pirogovskaya 1a, Moscow 119992, Russia

[5] Kurchatov Inst, Natl Res Ctr, Pl Akad Kurchatova 1, Moscow 123182, Russia

[6] Moscow Technol Univ, Inst Fine Chem Technol, Pr Vernadskogo 86, Moscow 119571, Russia

[7] Russian Acad Sci, Enikolopov Inst Synthet Polymer Mat, Ul Profsoyuznaya 70, Moscow 117393, Russia

来源：

BIOLOGICHESKIE MEMBRANY | 2017年 / 34卷 / 03期

关键词：

mass spectrometry; TOF-SIMS; quantitative analysis; Langmuir Blodgett films; ion beam etching; ION MASS-SPECTROMETRY; POLYMERS;

D O I：

10.7868/S0233475517020050

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Langmuir-Blodgett lipid films and plasma membranes of glioma cells were analyzed using time-of-flight secondary ion mass spectrometry (TOF-SIMS). Bi-3(+) primary ion beam was determined to be the most efficient in various experimental setups. TOF-SIMS was shown to be applicable for a quantitative analysis of model lipid structures as well as plasma membranes of glioma cells U87MG in vitro. A combination of atomic-force microscopy and scanning electron microscopy yielded the depth resolution of similar to 10-20 nm for cell surface scanning by primary ion beam.

引用

页码：215 / 222

页数：8

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