A reverse-phase liquid chromatography/mass spectrometry method for the analysis of high-molecular-weight fructooligosaccharides

被引：27

作者：

Harrison, Scott J. ^{[1
,2
]}

Fraser, Karl ^{[1
]}

Lane, Geoffrey A. ^{[1
]}

Villas-Boas, Silas ^{[2
]}

Rasmussen, Susanne ^{[1
]}

机构：

[1] AgResearch Ltd, Grasslands Res Ctr, Palmerston North 4442, New Zealand

[2] Univ Auckland, Sch Biol Sci, Auckland 1142, New Zealand

来源：

ANALYTICAL BIOCHEMISTRY | 2009年 / 395卷 / 01期

关键词：

MASS-SPECTROMETRY; PERENNIAL RYEGRASS; FRUCTAN METABOLISM; LOLIUM-PERENNE; DROUGHT STRESS; PERFORMANCE; CARBOHYDRATE; PLANTS; STEMS;

D O I：

10.1016/j.ab.2009.08.010

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Many important crop and forage plants accumulate polymeric water-soluble carbohydrates as fructooligosaccharides (or fructans). We have developed an improved method for the analysis of the full fructan complement in plant extracts based on porous graphitized carbon chromatography coupled to negative electrospray ionization mass spectrometry. By the use of profile data collection and multiple charge state ions, the effective mass range of the ion trap was extended to allow for the analysis of very high-molecular-weight oligosaccharides. This method allows the separation and quantification of isomeric fructan oligomers ranging from degree of polymerization (DP) 3 to DP 49. (C) 2009 Elsevier Inc. All rights reserved.

引用

页码：113 / 115

页数：3

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