Cloning and characterization of an acidic cytolysin cDNA from sea anemone Sagartia rosea

被引：22

作者：

Jiang, XY ^{[1
]}

Yang, WL ^{[1
]}

Chen, HP ^{[1
]}

Tu, HB ^{[1
]}

Wu, WY ^{[1
]}

Wei, JW ^{[1
]}

Wang, J ^{[1
]}

Liu, WH ^{[1
]}

Xu, AL ^{[1
]}

机构：

[1] Zhongshan Univ, Coll Life Sci, Dept Biochem, Open Lab Marine Funct Genom Natl High Tech Dev, Guangzhou 510275, Peoples R China

来源：

TOXICON | 2002年 / 40卷 / 11期

关键词：

Src I; sea anemone; Sagartia rosea; cloning; acidic cytolysin;

D O I：

10.1016/S0041-0101(02)00173-3

中图分类号：

R9 [药学];

学科分类号：

1007 ;

摘要：

A full-length cDNA of cytolysin (Src I) was isolated from the tentacle of Sagartia rosea (a representative species in China) by reverse transcription polymerase chain reaction. The cDNA with an open reading frame of 648 bp encodes a precursor protein of 216 amino acids, which contains a prepropeptide of 38 amino acids including a signal peptide of 19 amino acids and a propart of 19 amino acids. Lys-Pro at C-terminus of propart is a cleavage site for proline-endopeptidase-like protease. The mature cytolysin has a molecular mass of 19.6 kDa and a pI value of 4.8. Src I is an acidic cytolysin found in sea anemone and shares 75% amino acid sequence similarity to equinatoxin II (Eqt II). The predicted secondary structure of the mature cytolysin comprises 15% alpha-helix, 45% beta-sheet, and 40% random coil. The characteristic amphiphilic alpha-helix of cytolysin is located at the N-terminus of the processed Src I. (C) 2002 Elsevier Science Ltd. All rights reserved.

引用

页码：1563 / 1569

页数：7

共 24 条

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