In vitro plantlet regeneration system from rhizomes and mannose-binding lectin analysis of Polygonatum cyrtonema Hua.

被引：8

作者：

Zhao, Qi ^{[1
,2
]}

Wu, Chuanfang ^{[1
]}

Wang, Wenguo ^{[1
]}

Yuan, Shu ^{[1
]}

Bao, Jinku ^{[1
]}

Chen, Fang ^{[1
]}

机构：

[1] Sichuan Univ, Sch Life Sci, Chengdu 610064, Peoples R China

[2] Chengdu Univ, Fac Biotechnol Ind, Chengdu 610106, Peoples R China

来源：

PLANT CELL TISSUE AND ORGAN CULTURE | 2009年 / 99卷 / 03期

基金：

中国国家自然科学基金;

关键词：

Plantlet regeneration; Traditional Chinese medicine; Mannose-binding lectin; Polygonatum cyrtonema Hua; HUMAN-IMMUNODEFICIENCY-VIRUS; MOLECULAR-CLONING; APOPTOSIS;

D O I：

10.1007/s11240-009-9600-4

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Polygonatum cyrtonema Hua. lectins (PCLs) were extracted from plantlets regenerated from rhizome explants of P. cyrtonema. Rhizome explants demonstrated a high frequency of callus induction (72.5%) and adventitious shoots differentiation (83.7%) on Murashige Skoog (MS) medium supplemented with 2.0 mg l(-1) 2,4-dichlorophenoxyacetic acid and 1.0 mg l(-1) 6-benzyladenine. The adventitious shoots could root readily on 1/2 MS medium + 0.5 mg l(-1) alpha-naphthaleneacetic acid and regenerate plantlets with a survival rate of 75.0%. Regenerated rhizomes were freeze-dried, macerated and prepared for total RNAs and proteins extraction. The PCL gene was cloned and its expression level was measured by RT-PCR. Western blot using a lectin-specific antibody revealed a similar amount in regenerated rhizomes compared to wild rhizomes, Furthermore, lectin derived from regenerated rhizomes retained its ability to haemagglutinate rabbit blood cells.

引用

页码：269 / 275

页数：7

共 24 条

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