DNA from buccal swabs recruited by mail: Evaluation of storage effects on long-term stability and suitability for multiplex polymerase chain reaction genotyping

被引：264
|
作者：
Freeman, B [1 ]
Smith, N [1 ]
Curtis, C [1 ]
Huckett, L [1 ]
Mill, J [1 ]
Craig, IW [1 ]
机构：
[1] Inst Psychiat, SGDP Res Ctr, London SE5 8AF, England
来源：
BEHAVIOR GENETICS | 2003年 / 33卷 / 01期
关键词：
DNA extraction; DNA stability; multiplex polymerase chain reaction; sample storage;
D O I：
10.1023/A:1021055617738
中图分类号：
B84 [心理学]; C [社会科学总论]; Q98 [人类学];
学科分类号：
03 ; 0303 ; 030303 ; 04 ; 0402 ;
摘要：
We provide details of an inexpensive and rapid method for extraction of DNA from buccal swabs (including samples received through the mail) and from a range of other tissue samples. The procedure we have developed provides amounts of DNA adequate for several thousand polymerase chain reactions (PCRs), and we have validated its potential for long-term storage. Samples stored for >4 years are of comparable concentration and provide as robust PCR templates as those tested immediately after extraction. The availability of this technology is of considerable significance in planning DNA banks from population collections and cohorts.
引用
收藏
页码：67 / 72
页数：6
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