Simultaneous Measurements of Structure and Water Permeability in an Isolated Human Skin Stratum Corneum Sheet

被引：7

作者：

Nakazawa, Hiromitsu ^{[1
]}

Imai, Tomohiro ^{[1
]}

Suzuki, Mika ^{[1
]}

Akakabe, Natsuki ^{[1
]}

Hatta, Ichiro ^{[2
]}

Kato, Satoru ^{[1
]}

机构：

[1] Kwansei Gakuin Univ, Sch Sci & Technol, Sanda 6691337, Japan

[2] Nagoya Ind Sci Res Inst, Dept Res, Nagoya, Aichi 4600008, Japan

来源：

POLYMERS | 2019年 / 11卷 / 05期

关键词：

intercellular lipid; stratum corneum; transepidermal water loss; wide-angle X-ray diffraction; LIPID ORGANIZATION; CHAMBER SYSTEM; IN-VIVO; BARRIER; DIFFRACTION; HYDRATION; DOMAINS; MATRIX;

D O I：

10.3390/polym11050829

中图分类号：

O63 [高分子化学（高聚物）];

学科分类号：

070305 ; 080501 ; 081704 ;

摘要：

Stratum corneum (SC), the outermost layer of human skin, acts as an intelligent physicochemical interface between the inside and the outside of our body. To make clear the relationship between structure and physical barrier properties of SC, we developed a method that enables us to simultaneously acquire X-ray diffraction (XD) patterns and transepidermal water loss (TEWL) values using a spread SC sheet isolated from human skin. The synchrotron X-ray was incident on the SC sheet surface at an angle of 45 degrees to avoid interference between the two kinds of measurements. Detailed comparison between XD and TEWL data suggested that the thermal behavior of water permeability is closely related to the thermal expansion of the lattice spacings of the hexagonal phases above 40 degrees C and to the existence ratio of the orthorhombic phase below 40 degrees C. Thus, the new method we developed can give useful information on the mechanism of water permeation in SC without ambiguity caused by separate measurements of structure and water permeability with different samples.

引用

页数：11

共 29 条

[1] Molecular organization of the lipid matrix in intact Stratum corneum using ATR-FTIR spectroscopy [J].